Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,823 results for: golden gate

Clear keywords
Showing: 401 - 420 of 4823 results

  1. pICSL47761-LC

    Plasmid
    #245715
    Purpose
    Level 1 Position 4 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245715-plasmid-map-sequence-id-496040

    Enlarge

  2. pICSL47742-LC

    Plasmid
    #245713
    Purpose
    Level 1 Position 2 FORWARD acceptor Low - copy (with pBR322 origin of replication) GGAG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245713-plasmid-map-sequence-id-496035

    Enlarge

  3. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

    Enlarge

  12. p028g13-Gb1

    Plasmid
    #249632
    Purpose
    Clone 8xHis-residues in tandem with Gb1 polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3
    Depositor
    Isabel Abreu
    Article
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    N-terminal tag with 8x His-residues in tandem with Gb1 polypeptide
    Expression
    Bacterial
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    249632-plasmid-map-sequence-id-504978

    Enlarge

  14. p028g13-MBP

    Plasmid
    #249631
    Purpose
    Clone MBP polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3
    Depositor
    Isabel Abreu
    Article
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    N-terminal tag with MBP polypeptide
    Expression
    Bacterial
    Mutation
    Silent mutation on Gly-20 to remove a BsaI recogn…
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    249631-plasmid-map-sequence-id-504970

    Enlarge

  16. pNDGG005

    Plasmid
    #231309
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
    Expression
    Bacterial
    Available Since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231309-plasmid-map-sequence-id-472146

    Enlarge

  17. pNDGG006

    Plasmid
    #231310
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
    Expression
    Bacterial
    Available Since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231310-plasmid-map-sequence-id-463781

    Enlarge

  18. pNDGG007

    Plasmid
    #231311
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
    Expression
    Bacterial
    Available Since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231311-plasmid-map-sequence-id-463780

    Enlarge

Showing: 401 - 420 of 4823 results