We narrowed to 675 results for: intein

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pTXG-hXRCC1-K176R

Plasmid

#52284

Purpose

Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

XRCC1 (XRCC1 Human)

Tags

GyrA-intein-GST

Expression

Bacterial

Mutation

K176R, SUMO acceptor site mutant

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pTarget-Dual

Plasmid

#207884

Purpose

Carries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.

Depositor

David Edgell

Article

Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

Inserts

I-OnuI target site
I-GpeMI target site

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAXY0018

Plasmid

#205587

Purpose

p3flag_HygR(1-52)-NpuDnaE(N)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

HygR-NpuDnaE(N)_homo-codon-OP

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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OsMTI-1B Oryza sativa Metallothionein 1B

Plasmid

#200295

Purpose

Bacterial expression plasmids for production of recombinant Oryza sativa Metallothionein 1B

Depositor

Artur Krężel

Article

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein (LOC4332487 Oryza sativa)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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IBMc120

Plasmid

#161950

Purpose

Contains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB4A3-PECF20-B30-mScarlet-I-B15

Expression

Bacterial

Promoter

PECF20

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc393

Plasmid

#161953

Purpose

Demonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB4A3-T-PT7-B30-mScarlet-I-3T

Expression

Bacterial

Promoter

PT7

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc330

Plasmid

#161952

Purpose

Contains elements for building reporter plasmid for IBM. SrpR reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA171-PSrpR-B30-mScarlet-I-Ter

Expression

Bacterial

Promoter

PSrpR

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc259

Plasmid

#161951

Purpose

Contains elements for building reporter plasmid for IBM.TetR reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter

Expression

Bacterial

Promoter

PTet*

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc318

Plasmid

#161941

Purpose

Carries Golden Gate substitution insert for ER-LBD domain-insertion mapping

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-G-ER-LBD-S-SapI

Expression

Bacterial

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSC-hXRCC1-IntE2

Plasmid

#52276

Purpose

Bacterial expression of human XRCC1 C-terminally fused to GyrA intein, SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

XRCC1 (XRCC1 Human)

Tags

GyrA-intein-Ubc9-GST

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-ZpGal4DBD

Plasmid

#199199

Purpose

Insert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-ZpGal4DBD

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-p65Zp

Plasmid

#199200

Purpose

Insert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-p65Zp

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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IBMc396

Plasmid

#161954

Purpose

Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T

Expression

Bacterial

Promoter

PBAD

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBT44c

Plasmid

#122600

Purpose

BE PACE selection, express C-intein-Cas9-ugi fusion

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

intein-dCas9-ugi fusion

Use

Synthetic Biology

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)

Plasmid

#107137

Purpose

Dominant negative PKA cGAL effector for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

kin-2a(G310D)::SL2::GFP

Expression

Worm

Promoter

15xUAS-delta-pes-10(basal promoter)

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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His_Naa80d in pTYB12

Plasmid

#188455

Purpose

Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

Naa80 (NAA80 Human)

Tags

intein-CBD, 6xHis

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSC-IntE2

Plasmid

#52265

Purpose

Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linker

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

Ubc9 GST fusion (UBE2I Human, Synthetic)

Tags

GST and GyrA-intein

Expression

Bacterial

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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IBMc071

Plasmid

#161943

Purpose

Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T

Expression

Bacterial

Promoter

PBAD

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET-29_GSS-6xHis-SUMO-VidC-EGFP-FLAG

Plasmid

#241009

Purpose

VidC-EGFP construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGFP-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-29_GSS-6xHis-SUMO-VidC-mCherry-FLAG

Plasmid

#241010

Purpose

VidC-mCherry construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-mCherry-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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