We narrowed to 4,428 results for: erf

1. pCMV_SEPT9_i1 S12A S13A-msfGFP

   Plasmid

   #180337

   Purpose

   mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 S12A S13A

   Promoter

   CMV

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUMO GLTP

   Plasmid

   #170732

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

   Insert

   GLTP (GLTP Human)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

   Plasmid

   #166814

   Purpose

   Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1322R

   Promoter

   CMV

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

   Plasmid

   #166812

   Purpose

   Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H1276G

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E

   Plasmid

   #166813

   Purpose

   Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K1277E

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1461R

   Plasmid

   #164002

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E1461R

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry W1460A

   Plasmid

   #164001

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   W1460A

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry M1457A

   Plasmid

   #164000

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   M1457A

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry Y1445A

   Plasmid

   #163999

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Y1445A

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Jam4.3-Fc-His

    Plasmid

    #72082

    Purpose

    Expresses the extracellular region of the JAM-4, isoform 3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam4.3 (Igsf5 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Jam4.1-AP-His

    Plasmid

    #71955

    Purpose

    Expresses the extracellular region of the JAM-4, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam4.1 (Igsf5 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Jam4.3-AP-His

    Plasmid

    #71956

    Purpose

    Expresses the extracellular region of the JAM-4, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam4.3 (Igsf5 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. S4246

    Bacterial Strain

    #156372

    Purpose

    Erythromycin-resistant S2060 derivative

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Bacterial Resistance

    Streptomycin, tetracycline, erythromycin

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

14. pCMV_msfGFP-SEPT7

    Plasmid

    #180315

    Purpose

    mammalian expression of human SEPT7 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN7 (SEPTIN7 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_SEPT2-msfGFP

    Plasmid

    #180318

    Purpose

    mammalian expression of human SEPT2 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-idCas9-KRAB-Hygro donor

    Plasmid

    #199621

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Insert

    AAVS1-idCas9-KRAB-Hygro
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCK302

    Plasmid

    #87768

    Purpose

    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

    Inserts

    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    

    Use

    Synthetic Biology

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD rhamnose-inducible promoter from E. coli …

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid

    #188899

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187334

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF1330

    Plasmid

    #143950

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TFAP2C (TFAP2C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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