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EGFP-SopB

Plasmid

#183657

Purpose

N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-BX SpyCatcher003-hCD52

Plasmid

#210565

Purpose

Expresses Spycatcher003 on the cell surface using human CD52 hinge

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Spycatcher003 fused to human CD52 hinge

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-PDL1

Plasmid

#159280

Purpose

Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

Human PDL1/CD274 (CD274 Human)

Use

AAV; S1 knockin donor vector

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-acta2

Plasmid

#78686

Purpose

acta2 promoter for Gateway cloning

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Promoter

acta2

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Irf7

Plasmid

#178445

Purpose

doxycycline-inducible expression of Mouse Irf7 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Irf7 (Irf7 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-IRF5

Plasmid

#178450

Purpose

doxycycline-inducible expression of Human IRF5 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

IRF5 (IRF5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pbZIP9:GFP-GUS

Plasmid

#172483

Purpose

GFP-GUS driven by the bZIP9 promoter

Depositor

Wolf Frommer

Article

Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

Insert

bZIP9 promoter (BZIP9 Mustard Weed)

Tags

GFP and GUS

Expression

Plant

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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INS-2A-luciferase-2A-CD19-GFP

Plasmid

#159349

Purpose

Human INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Inserts

Luciferase-2A-human_CD19-2A-GFP
Luciferase-2A-human_CD19-2A-GFP (CD19 Human)

Use

Ins locus knockin donor vector

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-CA (Q67L)

Plasmid

#101050

Purpose

Expresses HA-tagged human Rab8-CA (Q67L) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-CREM

Plasmid

#178442

Purpose

doxycycline-inducible expression of Human CREM in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

CREM (CREM Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Zeo-Ezh2

Plasmid

#40794

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Ezh2 (Ezh2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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Teto-FUW-Pax6

Plasmid

#61541

Purpose

Expresses Pax6 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Pax6 (Pax6 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
TetO-FUW-MEF2CA

Plasmid

#61538

Purpose

Expresses MEF2CA in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

MEF2CA (MEF2C Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
FUW-TetO-Cdx2

Plasmid

#128829

Purpose

dox-dependent expression of mouse Cdx2

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Cdx2 (Cdx2 Mouse)

Use

Lentiviral

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTetON-DeAct-SpvB

Plasmid

#89455

Purpose

Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

TRE3G

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-DN (T22N)

Plasmid

#101049

Purpose

Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

T22N

Promoter

CMV

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Tead4

Plasmid

#128832

Purpose

dox-dependent expression of mouse Tead4

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Tead4 (Tead4 Mouse)

Use

Lentiviral

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

Plasmid

#126697

Purpose

donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

TagBFP

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126698

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

sgRNA targeting human SCGB3A2 locus

Expression

Mammalian

Promoter

Human U6

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pWPXL-Klf4

Plasmid

#36981

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 6, 2012

Availability

Academic Institutions and Nonprofits only