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pDONR223-MAP2K1IP1

Plasmid

#23694

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP2K1IP1 (LAMTOR3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DAK

Plasmid

#23870

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DAK (DAK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Lenti-EF1α-EGFP-WPRE

Plasmid

#250407

Purpose

Lentiviral expression of EGFP under EF1α core promoter.

Depositor

Stefan Kubicek

Article

Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR223-DGKB

Plasmid

#23477

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DGKB (DGKB Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-GK5

Plasmid

#23554

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GK5 (GK5 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DCAKD

Plasmid

#23585

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DCAKD (DCAKD Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-GLYCTK

Plasmid

#23653

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GLYCTK (GLYCTK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pSXL101

Plasmid

#247078

Purpose

pZE12-luc containing MNX1 from Candida parapsilosis CBS604 and ubiC from E. coli

Depositor

Yajun Yan

Article

Shen et al Metab Eng. 2017 Jul;42:52-58. doi: 10.10

Inserts

MNX1
ubiC
AS

Expression

Bacterial

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymNeongreen-SpHis5

Plasmid

#125704

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeonGreen

Tags

ymNeonGreen

Expression

Yeast

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-GCaMP6f

Plasmid

#127870

Purpose

Fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

4 repeats of COX8 signal peptide plus GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMSP2N2

Plasmid

#29520

Depositor

Stephen Sligar

Article

Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

Insert

MSP2N2

Tags

7-His tag

Expression

Bacterial

Mutation

tandem MSP; His-tag on N-terminus followed by spa…

Available Since

May 3, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-OMM-GCaMP6f

Plasmid

#127874

Purpose

Fluorescent reporter for mitochondrial outer membrane Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Outer membrane localized GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PP7_mCherry

Plasmid

#61763

Purpose

Expresses PP7 coat protein fused to mCherry

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

PP7 coat protein fused to mCherry

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

Ubc promoter

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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MS2_GFP

Plasmid

#61764

Purpose

Expresses MS2 coat protein fused to GFP

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

MS2 coat protein fused to eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

Ubc promoter

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pIVEX-SNAP-GFP

Plasmid

#46846

Purpose

SNAP-GFP fusion

Depositor

Florian Hollfelder

Article

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Insert

SNAP-GFP fusion

Use

In vitro expression

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pFGAMS-EGFP

Plasmid

#99107

Purpose

Expresses human PFAS-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

PFAS (PFAS Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pREDawn-AmpR-MCS

Plasmid

#188978

Purpose

For red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pIA1400

Plasmid

#172519

Purpose

Expression of SARS-COV-2 Nsp12 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BYG

Plasmid

#158126

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Cry2olig

Plasmid

#208780

Purpose

Inducibly expresses light activatable Cry2 domain

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cry2 mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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