-
Plasmid#110617PurposeBTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymeraseDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
SapTrap Kit
Plasmid Kit#1000000077PurposeSapTrap: modular toolkit for building CRISPR/Cas9 targeting vectors to insert genetic tags in C. elegans genome. 21 vector assembly plasmids, Unc-32::GFP targeting vector, 5 Cre/Flp expression vectorsDepositorAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCWXPG-UBI-PDX1
Plasmid#114304PurposeConstitutive expression of the PDX1 trancription factorDepositorInsertPDX1 (PDX1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a CHR2 mCherry WPRE
Plasmid#129432Purposeexpression of CHR2 mCherry from EF1a promoterDepositorInsertCHR2 mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-N-CretrcintG
Plasmid#69572PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cellsDepositorInsertkozak consensus - N-CretrcintG
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-C-CreintG
Plasmid#69573PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cellsDepositorInsertkozak consensus - C-CreintG
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGG198
Plasmid#165605PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFPDepositorInserthEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
UseSynthetic BiologyTagsExpressionBacterialMutationSecondary protospacer ('CGGCG' PAM) insā¦PromoterlacAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-H2GH
Plasmid#182202PurposeAdeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion constructDepositorInserthistone2B-HaloTag-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDK365
Plasmid#134195PurposeRetroviral plasmid with SKAP insertDepositorInsertSKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)
UseRetroviralTagsLAP (GFP)ExpressionMammalianMutationAlternate Start, G5 siRNA hardenedPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcNB1
Plasmid#89241PurposeResurfaced Cell-penetrating GFP nanobodyDepositorInsertpcNB1 (GFP)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMLS234
Plasmid#73682PurposeSapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable markerDepositorInsertGFP + Cbr-unc-119
UseCRISPR and Cre/LoxTagsGFPExpressionWormMutationPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEGO-GM55
Plasmid#217413PurposeLuciferase expression vector with the minimal GM-CSF promoterDepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterHuman -55 to +28 minimal CSF2 promoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRPN265NB-LaG16-MNase
Plasmid#170978PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/LaG16/MNase
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD282
Plasmid#66823PurposeGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized GFPExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pADsacA
Plasmid#158649PurposeConstitutive transcription of sacA crRNA and GFPDepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baciā¦TagsExpressionBacterialMutationPromoterPvegAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-tomm20-ECFP-RspANFAST
Plasmid#216348Purposetargets ECFP-RspANFAST to mitochondrial membraneDepositorInserttomm20-ECFP-RspANFAST
UseTagstomm20ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-tdTomato-miRE_shRen-Puro
Plasmid#135687PurposeConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCWXPG-UBI-PDX1:VP16
Plasmid#114305PurposeConstitutive expression of a PDX1:VP16 fusion proteinDepositorInsertPDX1-VP16 (Pdx1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-tdTomato-miRE_shRen-Blast
Plasmid#135686PurposeConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-Epo
Plasmid#114290PurposeTET-inducible expression of ErythropoietinDepositorInsertErythropoietin (Epo Rat)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only