We narrowed to 5,047 results for: IRES

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mCherry-NIP45 wt

Plasmid

#210266

Purpose

Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Tags

mCherry

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 wt

Plasmid

#210262

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

Plasmid

#174895

Purpose

amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

102TAG 150TAA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1

Plasmid

#197421

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2

Plasmid

#197422

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

Plasmid

#197423

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

Plasmid

#197424

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

Plasmid

#197425

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

Plasmid

#190154

Purpose

Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp3 (Vamp2 Rat)

Use

AAV

Tags

P2AT2A-EGFP-caax

Expression

Mammalian

Mutation

Truncation that includes only rat Vamp3 AA #1-81

Promoter

MBP

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-NMI

Plasmid

#178446

Purpose

doxycycline-inducible expression of Human NMI in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

NMI (NMI Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-BATF2

Plasmid

#178453

Purpose

doxycycline-inducible expression of Human BATF2 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

BATF2 (BATF2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-WW-CR-W3083F

Plasmid

#18076

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Mutation

Second conserved Tryptophan (W) of the WW domain …

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-CR-W3083F/P3086A

Plasmid

#18077

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Mutation

Second conserved Tryptophan (W) of the WW domain …

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-CR-d-ZZ-W3083F

Plasmid

#18078

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Mutation

Second conserved Tryptophan (W) of the WW domain …

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-CR-d-ZZ-W3083F/P3086A

Plasmid

#18079

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Mutation

Second conserved Tryptophan (W) of the WW domain …

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
EMMA toolkit

Plasmid Kit

#1000000119

Purpose

Used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated production

Depositor

Yizhi Cai

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only
pCMV-Vamp3-pHluorin

Plasmid

#190152

Purpose

Expresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosis

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp3 (Vamp3 Rat)

Use

AAV

Tags

superecliptic pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE4

Plasmid

#197148

Purpose

Expresses ApoE4 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE4 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE3

Plasmid

#197147

Purpose

Expresses ApoE3 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE3 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_ACh3.0

Plasmid

#121922

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Has Service

AAV9

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Promoter

hSyn

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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