Lysosomal trafficking of the glucose transporter GLUT1 requires sequential regulation by TXNIP and ubiquitin.

Qualls-Histed SJ, Nielsen CP, MacGurn JA
iScience. 2023 Feb 6;26(3):106150. doi: 10.1016/j.isci.2023.106150. eCollection 2023 Mar 17. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
200085	WT WWP1	WWP1 in pQCXIN vector
200086	WT Nedd4L	Nedd4L in pQCXIN vector
200087	E228Q Vps4A-HA	3x HA-VPS4A with E228Q dominant negative mutation in Dox-inducible pInducer20 vector
200088	WT TXNIP	Dox-inducible WT human TXNIP in pInducer20 vector
200090	TXNIP-py	Dox-inducible human TXNIP with py motif mutations (ppcy,ppty -->paca,pata) in pInducer20 vector
200091	WT TXNIP-FLAG	WT human TXNIP with C-terminal 3xFLAG tag in pQCXIP vector
200092	TXNIP-py-FLAG	TXNIP py motif mutant with C-terminal 3xFLAG tag in pQCXIP vector
200093	WT GLUT1-GFP	WT human GLUT1 with a C-terminal GFP tag in pQCXIP vector
200094	WT GLUT1-FLAG	WT human GLUT1 with a C-terminal 3xFLAG tag in pQCXIP vector
200095	1K245-->R GLUT1-GFP	1K245--> GLUT1 mutant with C-terminal GFP tag in pQCXIP vector
200096	11K-cyto-->R GLUT1-GFP	human GLUT1 with K-->R mutations for all 11 cytosolic lysines and a C-terminal GFP tag in pQCXIP vector
200097	1K245 GLUT1-GFP	human GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vector
200098	6K-loop-->R GLUT1-GFP	human GLUT1 with K-->R mutation of 6 lysines in the major cytoplasmic loop and C-terminal GFP tag in pQCXIP vector
200099	5K-tails-->R GLUT1-GFP	human GLUT1 with K-->R mutation of 5 lysines on the N- and C-terminal tails with a C-terminal tag in pQCXIP vector