Lysosomal trafficking of the glucose transporter GLUT1 requires sequential regulation by TXNIP and ubiquitin.
Qualls-Histed SJ, Nielsen CP, MacGurn JA
iScience. 2023 Feb 6;26(3):106150. doi: 10.1016/j.isci.2023.106150. eCollection 2023 Mar 17. PubMed Article
Qualls-Histed SJ, Nielsen CP, MacGurn JA
iScience. 2023 Feb 6;26(3):106150. doi: 10.1016/j.isci.2023.106150. eCollection 2023 Mar 17. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200085 | WT WWP1 | WWP1 in pQCXIN vector |
| 200086 | WT Nedd4L | Nedd4L in pQCXIN vector |
| 200087 | E228Q Vps4A-HA | 3x HA-VPS4A with E228Q dominant negative mutation in Dox-inducible pInducer20 vector |
| 200088 | WT TXNIP | Dox-inducible WT human TXNIP in pInducer20 vector |
| 200090 | TXNIP-py | Dox-inducible human TXNIP with py motif mutations (ppcy,ppty -->paca,pata) in pInducer20 vector |
| 200091 | WT TXNIP-FLAG | WT human TXNIP with C-terminal 3xFLAG tag in pQCXIP vector |
| 200092 | TXNIP-py-FLAG | TXNIP py motif mutant with C-terminal 3xFLAG tag in pQCXIP vector |
| 200093 | WT GLUT1-GFP | WT human GLUT1 with a C-terminal GFP tag in pQCXIP vector |
| 200094 | WT GLUT1-FLAG | WT human GLUT1 with a C-terminal 3xFLAG tag in pQCXIP vector |
| 200095 | 1K245-->R GLUT1-GFP | 1K245--> GLUT1 mutant with C-terminal GFP tag in pQCXIP vector |
| 200096 | 11K-cyto-->R GLUT1-GFP | human GLUT1 with K-->R mutations for all 11 cytosolic lysines and a C-terminal GFP tag in pQCXIP vector |
| 200097 | 1K245 GLUT1-GFP | human GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vector |
| 200098 | 6K-loop-->R GLUT1-GFP | human GLUT1 with K-->R mutation of 6 lysines in the major cytoplasmic loop and C-terminal GFP tag in pQCXIP vector |
| 200099 | 5K-tails-->R GLUT1-GFP | human GLUT1 with K-->R mutation of 5 lysines on the N- and C-terminal tails with a C-terminal tag in pQCXIP vector |