We narrowed to 4,424 results for: 187

1. S2 1058 V82G ABEMax

   Plasmid

   #135361

   Purpose

   Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   1058 nSpyCas9 V82G ABEMax
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. S1 1029 V82G ABEMax Strategy 3

   Plasmid

   #135363

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   1029 V82G ABEMax
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. miniABEMax V82G nSaCas9

   Plasmid

   #135365

   Purpose

   Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   miniABEMax V82G nSaCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. AIDx-ID-202

   Plasmid

   #135344

   Purpose

   Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 202 of nSpyCas9 used for base editing

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx-ID-202
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRC/CMV-TYK2(delta)TK-VSV

   Plasmid

   #139353

   Purpose

   Expression of TYK2 protein missing the tyrosine kinase (TK) domain (aa 1-895)

   Depositor

   Sandra Pellegrini

   Article

   Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

   Insert

   TYK2 cDNA expressing aa 1-895 (TYK2 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   V362F-deletion of aa 896-1187

   Promoter

   CMV

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-X1-Neo-PMP34-DDRGK1-dTM-HA

   Plasmid

   #139862

   Purpose

   Lentiviral expression of PMP34-DDRGK1-dTM-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   PMP34-DDRGK1-dTM (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   recombinant fusion of PMP34 (full length) with DD…

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCRII apoBa

   Plasmid

   #121897

   Purpose

   Plasmid for making zebrafish apoBa in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   ApoBa (apoba Zebrafish)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TPD52L3 gRNA (BRDN0001147599)

   Plasmid

   #76953

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TPD52L3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TPD52L3 (TPD52L3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. TPD52L3 gRNA (BRDN0001144944)

   Plasmid

   #76954

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TPD52L3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TPD52L3 (TPD52L3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PA-mCherry-miniSOG-Keratin-17

    Plasmid

    #57792

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. mCherry-miniSOG-Keratin-17

    Plasmid

    #55091

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. KHBD00060

    Plasmid

    #34221

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9415 (Xbp1 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

13. BII-CMV-AfmW3A

    Plasmid

    #133402

    Purpose

    Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Afamin and Wnt3a (Afm, Wnt3a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216246

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgAAVS1.5
    

    Use

    CRISPR; Human safe harbor locus (a avs1) site-spe…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHRIG-Akt1

    Plasmid

    #53583

    Purpose

    lentiviral expression of constitutively active human Akt1 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    cAkt1 (AKT1 Human)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available since

    Oct. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Villinpromoter-blue-FlpOERT2

    Plasmid

    #67278

    Purpose

    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

    Insert

    Villin-mTQ2-P2A-FlpO-ERT2
    

    Use

    Tags

    mTQ2, linked to FlpO through an P2A ribosomal ski…

    Expression

    Mammalian

    Mutation

    Promoter

    Villin

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

    Plasmid

    #216244

    Purpose

    Express AtAFB2.F74A mutant with mCherry tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    AtAFB2.F74A-mCherry
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F74A mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTMB352_ZFcharm_Kv1_split-inteins

    Plasmid

    #220848

    Purpose

    Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv2
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. ADAM10-bio-His

    Plasmid

    #51641

    Purpose

    Expresses full-length ADAM10 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

    Plasmid

    #154910

    Purpose

    1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.

    Depositor

    Jonathan Clarke, Clare Buckley

    Article

    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

    Insert

    Phytochrome B (PHYB Synthetic, Mustard Weed)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    CAAX membrane moiety and mCherry

    Expression

    Mammalian

    Mutation

    1-621 amino acids of Arabidopsis PHYB

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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