We narrowed to 10,674 results for: cat.1

1. Desmoplakin II no force control (FL-based)

   Plasmid

   #118724

   Purpose

   The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-FL-mCherry

   Expression

   Mammalian

   Mutation

   truncation after aa1353

   Promoter

   CMV

   Available since

   Dec. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1494

   Plasmid

   #142393

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOSL1 (FOSL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKLV2-U6gRNA5(NR5A2_5-2)-PGKpuroBFP-W

   Plasmid

   #211976

   Purpose

   Express gRNA against NR5A2 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting NR5A2 (NR5A2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2816

   Plasmid

   #144245

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOSB (FOSB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TFORF1492

   Plasmid

   #144179

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOSL1 (FOSL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF2313

   Plasmid

   #142404

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ETV3 (ETV3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT22UbGfpnE4PCh(#301)

   Plasmid

   #184066

   Purpose

   cyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Zhang et al Chembiochem. 2018 Jun 18;19(12):1232-123

   Insert

   eGFP-nls-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-N-Myc_1-560 USP7

   Plasmid

   #131244

   Purpose

   Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Expresses USP7 amino acids 1 - 560. This truncati…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

   Plasmid

   #173029

   Purpose

   For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

   Depositor

   Mohamed Fareh

   Article

   Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

   Insert

   pspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, BFP, HIV NES, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-dCTNNA1

    Plasmid

    #176032

    Purpose

    A knock-out vector for dog CTNNA1 (alpha-1-catenin)

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog CTNNA1 (alpha-1-catenin) gene and the cDNA of Cas9 (CTNNA1 )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTetOne-human cGASdeltaN-HA

    Plasmid

    #186897

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (Adamts1 Synthetic, Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRE3G-human cGASdeltaN-HA

    Plasmid

    #186843

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (Adamts1 Synthetic, Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTRE3G-HA-human cGASdeltaN

    Plasmid

    #186852

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS truncation mutant (160-522 a.a.) with N-terminal HA tag (Adamts1 Synthetic, Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTRE3G-human cGASdeltaN

    Plasmid

    #186854

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS truncation mutant (160-522 a.a.) without tags (Adamts1 Synthetic, Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

    Plasmid

    #153440

    Purpose

    E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc. XDocB binding mode A knock-out mutant
    

    Use

    Tags

    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

    Expression

    Bacterial

    Mutation

    Arginine 191 and leucine 195 on XDocB were mutate…

    Promoter

    T7 promoter

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ZO-2 shRNA 1320

    Plasmid

    #37210

    Depositor

    James Anderson, Alan Fanning

    Article

    Van Itallie et al Mol Biol Cell. 2009 Sep;20(17):3930-40.

    Insert

    dog ZO-2 shRNA (TJP2 canine)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pTaU6-esgRNA

    Plasmid

    #115630

    Purpose

    expression esgRNA in wheat protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    TaU6p-esgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOsU3-esgRNA

    Plasmid

    #115629

    Purpose

    expression esgRNA in rice protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    OsU3p-esgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. T7-LacO-TetR_p15A

    Plasmid

    #171664

    Purpose

    Expression of TetR in BL21(DE3) E. coli. Contains p15A origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-LacO-TetR-T7 Stop
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. T7-LacO-QF_p15A

    Plasmid

    #171651

    Purpose

    Expresses QF activator protein in BL21(DE3) E. coli. Contains p15A origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QF-T7 Stop
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge