We narrowed to 30,544 results for: ide

1. pGEMT-Mef2c-tPT2A-GFP

   Plasmid

   #111771

   Purpose

   Bicistronic construct: Co-express two genes by 2A peptides

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Insert

   Mef2c-tPT2A-GFP (Mef2c Mouse)
   

   Use

   Cloning intermediate

   Tags

   GFP

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET14b His Rab8a Q67L

   Plasmid

   #186014

   Purpose

   His Rab8a Q67L

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   His Rab8a Q67L (RAB8A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-HyPer-DAAO-NES

   Plasmid

   #119164

   Purpose

   Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.

   Depositor

   Thomas Michel

   Article

   Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

   Insert

   HyPer-DAAO-NES
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPRKCZ-C1-EGFP (C-PRKCZ-C1)

   Plasmid

   #217759

   Purpose

   Fluorescent reporter for ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Protein kinase C zeta (PRKCZ Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C1 domain (aa 123-193)

   Promoter

   CMV

   Available since

   June 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

   Plasmid

   #138146

   Purpose

   Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PspCas13b
   

   Use

   Lentiviral

   Tags

   NES

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBADHISTEV_PfeE

   Plasmid

   #129120

   Purpose

   pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

   Depositor

   James Naismith

   Article

   Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

   Insert

   Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
   

   Use

   Tags

   TEV protease cleavable 6xHis

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   PBAD

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EGFR-ET1

   Plasmid

   #44185

   Depositor

   Timothy Springer

   Article

   Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

   Insert

   EGFR-ET1 (EGFR Human)
   

   Use

   Tags

   3C cleavge site, protein C peptide tag, his6, SBP…

   Expression

   Mammalian

   Mutation

   I104V and D254N relative to NP_005219.2

   Promoter

   CMV

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET20b_Pa-PiuA

   Plasmid

   #128955

   Purpose

   expresses Pseudomonas aeruginosa PiuA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
   

   Use

   Tags

   6xHis and pelB leader

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330-Flag-deSpCas9

   Plasmid

   #92114

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2-mkgDUX4

    Plasmid

    #21156

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed

    Plasmid

    #157974

    Purpose

    Template plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9

    Depositor

    Carolyn McBride

    Article

    Zhao et al Nature. 2022 May;605(7911):706-712. doi:

    Insert

    Orco
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET20b_Pa-PirA

    Plasmid

    #128956

    Purpose

    expresses Pseudomonas aeruginosa PirA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    ferric enterobactin receptor PirA (pirA Synthetic, Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRF+423Dux4

    Plasmid

    #21625

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    Double homeobox, chr 4 (DUX4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

    Plasmid

    #138144

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NES

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDECKO_mCherry_non-targeting

    Plasmid

    #140109

    Purpose

    CRISPR-Cas9 library validation (negative control)

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26

    Plasmid

    #201685

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26

    Plasmid

    #201686

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1 (V106W)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330-Flag-dSpCas9-HF1

    Plasmid

    #92115

    Purpose

    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1P POLD1_2

    Plasmid

    #160792

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge