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pDropIn-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199196

Purpose

Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed

Expression

Insect

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

Plasmid

#199197

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int-BlastR

Expression

Insect

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

Plasmid

#171102

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hSpCas9 (Otx2 Mouse, S. pyogenes)

Use

CRISPR

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

Plasmid

#171101

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hSpCas9 (Olig2 Mouse, S. pyogenes)

Use

CRISPR

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRRLsin.PPT.CMV‐GFP‐Eps8 WT

Plasmid

#74922

Purpose

Lentiviral plasmid expressing Eps8 fused to GFP

Depositor

Giorgio Scita

Publication

Menna et al PLoS Biol. 2009 Jun 30;7(6):e1000138. do

Insert

Eps8 (Eps8 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
pT/Caggs-NRAS G12V/D38A

Plasmid

#221073

Purpose

Expresses NRAS G12V/D38A in mammalian cells

Depositor

Hidenori Ichijo

Publication

Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

Insert

NRAS (NRAS Human)

Expression

Mammalian

Mutation

G12V/D38A

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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CMV SNAP-Synapsin

Plasmid

#206152

Purpose

CMV SNAP-Synapsin

Depositor

Erika Holzbaur

Publication

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Synapsin

Tags

SNAPf

Expression

Mammalian

Promoter

CMV

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pVG49

Plasmid

#164707

Purpose

yEVenus under the control of GAL1 promoter

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

GAL1pr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+mTurquoise2-Golgi

Plasmid

#128425

Purpose

Contains insert allowing in-vivo visualization of Golgi apparatus

Depositor

Rusty Lansford

Publication

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mTurquoise2-Golgi

Expression

Mammalian

Promoter

CMV & SP6

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1818_pCR8-gRG6-SA

Plasmid

#118652

Purpose

Transient transfection; Expressed RG6-SA CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWT055f

Plasmid

#96865

Purpose

sgRNA-FANCF

Depositor

David Liu

Publication

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-FANCF

Expression

Mammalian

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT055g

Plasmid

#96864

Purpose

sgRNA-HEK3

Depositor

David Liu

Publication

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA-HEK3

Expression

Mammalian

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pSK32

Plasmid

#72854

Purpose

template for C-terminal TEV-site + V5-tag, Puromycin-Resistance

Depositor

Klaus Foerstemann

Publication

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

TEV-V5 tag

Expression

Insect

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDpy-7::oxCerulean-oxVenus::Lgg-1

Plasmid

#68042

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in hypodermis, for monitoring autophagy

Depositor

Dana Miller

Publication

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pDpy-7::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pYes2-TaPCS1

Plasmid

#49767

Purpose

Expresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Publication

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

TaPCS1 (PCS1 Triticum aestivum)

Expression

Yeast

Promoter

GAL1

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pYes2-AtPCS1

Plasmid

#49766

Purpose

Expresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Publication

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

AtPCS1 (PCS1 Mustard Weed)

Expression

Yeast

Promoter

GAL1

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab7 WT

Plasmid

#37699

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pUASP dsRed Rab5 DN

Plasmid

#44823

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

S43N (dominant negative)

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab11 WT

Plasmid

#37711

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab5 CA

Plasmid

#37702

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q88L (constitutive active)

Promoter

GAL4

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only