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pAAV_CAG(del)-FLEx(loxP)-mCherry(nuclear)-2A-N2cG

Plasmid

#248663

Purpose

Helper virus for monosynaptic tracing; Expresses nuclear mCherry and Rabies Glycoprotein (G) of CVS-N2c strain in Cre-expressing cells under the CAG promoter.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Inserts

N2c(G)
nuc-mCherry

Use

AAV

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-KIKO-U6-gThy1-p2a-mCherry-STOP-pA-400

Plasmid

#249129

Purpose

all-in-one HDRT-based knockin-knockout (KIKO) design to insert mCherry (=knockin) at gThy1 cut site, disrupting Thy1 expression (=knockout)

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gThy1, mCherry (Thy1 Synthetic, Mouse)

Use

AAV

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-0.5Syn-CasRX-mCherry-pA

Plasmid

#192491

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

0.5Syn

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DjCas13d-pA

Plasmid

#192495

Purpose

To express DjCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-CasRx-pA

Plasmid

#192487

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgNT-2-EF1a-LibVec

Plasmid

#239593

Purpose

expresses non-targeting (NT) guide#2

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

NT (non-targeting guide)

Use

AAV; To deliver a non-targeting guide (serving as…

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgNT-1-EF1a-LibVec

Plasmid

#239592

Purpose

expresses non-targeting (NT) guide#1

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

NT (non-targeting guide)

Use

AAV; To deliver a non-targeting guide (serving as…

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-miRFP670

Plasmid

#220600

Purpose

Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

miRFP670

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hIsl2-EGFP

Plasmid

#153206

Purpose

Human Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mIsl2-EGPF

Plasmid

#153191

Purpose

Mouse Isl2 (mIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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p-AAV2-hSyn-MerMAID1-Citrine

Plasmid

#126520

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID1

Use

AAV

Tags

Citrine

Promoter

human synapsin

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gCxl5-CBh-mCherry-SV40late

Plasmid

#249120

Purpose

expression of gCxcl5 and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gCxcl5 (Cxcl5 Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gOsmr-CBh-mCherry-SV40late

Plasmid

#249122

Purpose

expression of gOsmr and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gOsmr (Osmr Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gPdpn-CBh-mCherry-SV40late

Plasmid

#249123

Purpose

expression of gPdpn and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gPdpn (Pdpn Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gTgfbr2-CBh-mCherry-SV40late

Plasmid

#249125

Purpose

expression of gTgfbr2 and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gTgfbr2 (Tgfbr2 Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gTnfrfs1a-CBh-mCherry-SV40late

Plasmid

#249127

Purpose

expression of gTnfrsf1a and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gTnfrsf1a (Tnfrsf1a Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gTrac-CBh-mCherry-SV40late

Plasmid

#249128

Purpose

expression of gTrac and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gTrac (Trac Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gCtnnb1-CBh-mCherry-SV40late

Plasmid

#249119

Purpose

expression of gCtnnb1 and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gCtnnb1 (Ctnnb1 Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pOTTC2234 - pAAV Nestin GFP-iCre

Plasmid

#228443

Purpose

An adeno-associated viral vector to express GFP-iCre fusion protein under the human Nestin 2nd intron enhancer.

Depositor

Christopher Richie

Article

Ribeiro Gomes et al Cell Rep. 2025 Dec 21;45(1):116756. doi:

Insert

GFP-iCre

Use

AAV

Expression

Mammalian

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ECS-dSpyCatcher003

Plasmid

#234963

Purpose

Cre-dependent ECS-facing destabilized SpyCatcher003 (facing the extracellular space)

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-dSC

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

None

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-tdTomato-WPRE

Plasmid

#187112

Purpose

FLP-dependent expression of tdTomato under EF1a promoter

Depositor

Yongling Zhu

Article

Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

Insert

tdTomato

Use

AAV

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn1 CaRhAC T2A tDimer

Plasmid

#101722

Purpose

- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

Depositor

Thomas Oertner

Article

Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

Inserts

CatRhAC
red fluorescent protein

Use

AAV

Mutation

E497K,C569D

Promoter

human Synapsin1 promotor and ribosomal skip seque…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_CRF0.5

Plasmid

#208657

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5

Use

AAV

Promoter

hSyn

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-pCAG-Floxed-FlpO-2A-H2BV5c.o.,rev.-WPRE

Plasmid

#234894

Purpose

CAG-driven, Cre-dependent FlpO recombinase for H2B-V5

Depositor

Silvia Arber

Article

Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

Insert

Floxed-FlpO-2A-H2BV5

Use

AAV

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-miR122 TS

Plasmid

#209783

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter, incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

miR122 ts

Use

AAV

Tags

HA

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Pgc-1alpha-EYFP

Plasmid

#241793

Purpose

Expression vector for mouse Pgc-1alpha with EYFP

Depositor

Mary Kay Lobo

Article

Chandra et al Biol Psychiatry. 2017 Apr 1;81(7):564-57

Insert

PGC-1alpha (Ppargc1a Mouse)

Use

AAV

Tags

EYFP

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

Plasmid

#220610

Purpose

Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

FLAG-KORD-P2A-ArgiNLS-AausFP1

Use

AAV and Cre/Lox

Tags

FLAG; ArgiNLS

Expression

Mammalian

Promoter

hSyn

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV CTG uGIP polyGly (exon 4 Cter) GFP (OPDM type 2)

Plasmid

#250410

Purpose

GFP-tagged polyglycine protein expressed from an upstream small ORF of GIPC1. This vectors uses 100 GGN optimized codons. Translation is driven by a CTG near cognate start codon.

Depositor

Nicolas Charlet-Berguerand

Insert

uGIPpolyG

Use

AAV

Tags

GFP

Expression

Mammalian

Available Since

March 16, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pscAAV-U6-gSCR-CBh-mCherry-SV40late

Plasmid

#249124

Purpose

expression of gSCR (scrambled, non-targeting gRNA) and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gSCR (Scr Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV TRE-Tight CheRiff-mKate2

Plasmid

#182493

Purpose

Expression of CheRiff channelrhodopsin linked to mKate2 from a Tet-inducible promoter

Depositor

Thomas Oertner

Article

Lamothe-Molina et al Nat Commun. 2022 Oct 26;13(1):6376. doi:

Insert

CheRiff (#51697 Adam Cohen Lab)

Use

AAV

Tags

mKate2 (Evrogen)

Expression

Mammalian

Promoter

TRE-Tight (Clontech)

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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