We narrowed to 4,644 results for: LUM

1. pRATIO1267

   Plasmid

   #141301

   Purpose

   Gateway cloning compatible dual bioluminescent ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Use

   Tags

   gLUC and redLUC

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQIq_MRGS_HIS8_(27G2)_FLAG

   Plasmid

   #199616

   Purpose

   Encodes DARPin-FLAG 27G2 for bacterial expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-FLAG 27G2
   

   Use

   Tags

   8xHis and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-ER-rsTagRFP

   Plasmid

   #87620

   Purpose

   Endoplasmic reticulum (ER)-targeted rsTagRFP.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   ER-rsTagRFP
   

   Use

   Tags

   N-terminal 27 amino acids from CYP450

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

   Plasmid

   #177434

   Purpose

   To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

   Depositor

   David Andrews

   Insert

   Cb5 tail anchor (Cyb5r4 Rat)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAPV-DsRed2-FLAG-ER-5

   Plasmid

   #170622

   Purpose

   Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   APV-DsRed2-ER-5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-PRLSS-SH2-KDEL

   Plasmid

   #209716

   Purpose

   Mammalian expression of SH2 localized to endoplasmic reticulum

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   PRLSS-SH2-KDEL
   

   Use

   Tags

   PRLSS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQIq_MRGS_HIS8_( E3_5)_FLAG

   Plasmid

   #199612

   Purpose

   Encodes DARPin-FLAG E3_5 for bacterial expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-FLAG E3_5 (control)
   

   Use

   Tags

   8xHis and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pICSL50016

   Plasmid

   #50315

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   Codon-optimised for A. thaliana. BsaI/BbsI sites …

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Venus-7aa-Cb5-pEGFP-C1

   Plasmid

   #166764

   Purpose

   To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.

   Depositor

   David Andrews

   Insert

   Cb5 tail anchor (Cyb5a Rat)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mPapaya-CytERM-N-17

    Plasmid

    #56345

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    CytERM
    

    Use

    Tags

    mPapaya

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

11. mCerulean3-CytERM-N-17

    Plasmid

    #55412

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    CytERM
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mTFP1-CytERM-N-17

    Plasmid

    #55476

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    CytERM
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. BLA_CAT_K136_GPG_LOV2

    Plasmid

    #231575

    Purpose

    This plasmid mediates chloramphenicol resistance in the dark, resulting in Cm mediated cell death upon illumination with blue light

    Depositor

    Dominik Niopek

    Article

    Wolf et al bioRxiv 2024.12.04.626757

    Insert

    CAT_ carrying an AsLOV2 insertion behind K136
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUCmini-RELA-PGK-EGFP

    Plasmid

    #228051

    Purpose

    Homologous donor template for RELA knockout expressing EGFP

    Depositor

    Yohei Sato

    Article

    Sato et al Clinical Immunology Communications, Volu

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUCmini-RELB-PGK-EGFP

    Plasmid

    #228052

    Purpose

    Homologous donor template for RELB knockout expressing EGFP

    Depositor

    Yohei Sato

    Article

    Sato et al Clinical Immunology Communications, Volu

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUCmini-RELC-PGK-EGFP

    Plasmid

    #228053

    Purpose

    Homologous donor template for RELC knockout expressing EGFP

    Depositor

    Yohei Sato

    Article

    Sato et al Clinical Immunology Communications, Volu

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUCmini-RELA-PGK-tdTomato

    Plasmid

    #228054

    Purpose

    Homologous donor template for RELA knockout expressing tdTomato

    Depositor

    Yohei Sato

    Article

    Sato et al Clinical Immunology Communications, Volu

    Insert

    tdTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUCmini-RELC-PGK-tdTomato

    Plasmid

    #228056

    Purpose

    Homologous donor template for RELC knockout expressing tdTomato

    Depositor

    Yohei Sato

    Article

    Sato et al Clinical Immunology Communications, Volu

    Insert

    tdTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-PRLSS-yATOM_SH2-KDEL

    Plasmid

    #209714

    Purpose

    Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-yATOM_SH2-KDEL
    

    Use

    Tags

    PRLSS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL

    Plasmid

    #209718

    Purpose

    Mammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-NbGFP_miRFP670nano3-KDEL
    

    Use

    Tags

    PRLSS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge