We narrowed to 9,752 results for: try

1. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

   Plasmid

   #223065

   Purpose

   Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
   

   Use

   CRISPR

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Mutation

   Three regions of SpCas9 encode type IIS restricti…

   Promoter

   Dual T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4-eIF3B

   Plasmid

   #133308

   Purpose

   Expression of luciferase driven by eIF3B promoter region

   Depositor

   Josep Domingo-Domenech

   Article

   Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

   Insert

   eIF3B promoter (EIF3B Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   eIF3B

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

   Plasmid

   #237480

   Purpose

   Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.

   Depositor

   Dirk Daelemans

   Article

   Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

   Insert

   SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
   

   Use

   Lentiviral

   Tags

   IRES-mNeonGreen-NES/PKI and P2A-Blasticidin

   Expression

   Mammalian

   Mutation

   S484D + S813I + deleted amino acids 68-76 + 676-6…

   Promoter

   CMV

   Available Since

   July 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin

   Plasmid

   #237479

   Purpose

   Encodes full-length human TMEM106B. M210A/F213A mutations block S1 binding and SARS-CoV-2 Belgium/GHB-03021/2020 TMEM106B mediated infection, without affecting TMEM106B expression or localization.

   Depositor

   Dirk Daelemans

   Article

   Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

   Insert

   TMEM106B (TMEM106B Human)
   

   Use

   Lentiviral

   Tags

   P2A-Blasticidin

   Expression

   Mammalian

   Mutation

   M210A + F213A

   Promoter

   CMV

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4-eIF3B MBE3 mutant

   Plasmid

   #133310

   Purpose

   Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

   Depositor

   Josep Domingo-Domenech

   Article

   Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

   Insert

   eIF3B promoter MBE3 mutant (EIF3B Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   ccacgtgacc changed to cAaAAAAacc

   Promoter

   eIF3B

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4-eIF3B MBE2 mutant

   Plasmid

   #133309

   Purpose

   Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

   Depositor

   Josep Domingo-Domenech

   Article

   Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

   Insert

   eIF3B promoter MBE2 mutant (EIF3B Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   gccacatgcacc changed to gcAaAaAAcacc

   Promoter

   eIF3B

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

   Plasmid

   #202553

   Purpose

   Nontargeting control vector with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Mutation

   Puromycin-resistance gene in the pLV hU6-sgRNA hU…

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. MultiSite Gateway plasmid toolKit

   Plasmid Kit

   #1000000050

   Purpose

   Cloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modules

   Depositor

   Tomas Santalucia

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

9. RAS mutant clone collection

   Plasmid Kit

   #1000000089

   Purpose

   Wild-type and mutant HRAS, NRAS, KRAS4a and KRAS4b gene plasmids as Gateway entry clones

   Depositor

   Dominic Esposito

   Available Since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

10. Fungal Toolkit for Modular Cloning (FTK)

    Plasmid Kit

    #1000000191

    Purpose

    Consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native promoters, terminators, selection markers, and CRISPR components, for filamentous fungi.

    Depositor

    Arnold Driessen

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

11. E. coli K-12 MG1655_rLP5(nth-ydgR)

    Bacterial Strain

    #110245

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

12. TB205 △trpC

    Bacterial Strain

    #230038

    Purpose

    Fluorescently labelled (mCherry) E. coli, auxotrophic for tryptophan

    Depositor

    Olga Schubert, Martin Ackermann

    Article

    Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

    Bacterial Resistance

    None

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

13. TB204 △trpC

    Bacterial Strain

    #230037

    Purpose

    Fluorescently labelled (GFP) E. coli, auxotrophic for tryptophan

    Depositor

    Olga Schubert, Martin Ackermann

    Article

    Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

    Bacterial Resistance

    None

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

14. E. coli K-12 MG1655_fLP4(essQ-cspB)

    Bacterial Strain

    #110244

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

15. E. coli K-12 MG1655_rLP6(ygcE-ygcF)

    Bacterial Strain

    #110246

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

16. E. coli K-12 MG1655_rLP1(atpI-gidB)

    Bacterial Strain

    #110241

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the atpl-gidB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

17. E. coli K-12 MG1655_fLP3(ybbD-ylbG)

    Bacterial Strain

    #110243

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the ybbD-ylbG intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

18. E. coli K-12 MG1655_fLP2(yieN-trkD)

    Bacterial Strain

    #110242

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the yieN-trkD intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

19. TB204

    Bacterial Strain

    #230033

    Purpose

    Fluorescently labelled (monomeric sfGFP) E. coli

    Depositor

    Tobias Bergmiller, Calin Guet

    Article

    Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

    Bacterial Resistance

    None

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

20. ATMW-BL21

    Bacterial Strain

    #218769

    Purpose

    E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

    Bacterial Resistance

    Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

    Species

    Escherichia coli

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only