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pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

Plasmid

#185914

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Insert

human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pH-nCas9-PPE-V2

Plasmid

#170131

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

Insert

nCas9(H840A)-M-MLV

Use

CRISPR

Expression

Plant

Mutation

H840A for Cas9; D200N, T306K, W313F, T330P and L…

Promoter

maize Ubiquitin-1, OsU3

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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CaMKAR

Plasmid

#205315

Purpose

Fluorescent Reporter for CaMKII Activity

Depositor

Mark Anderson, Elizabeth Luczak

Article

Reyes Gaido et al Sci Transl Med. 2023 Jun 21;15(701):eabq

Insert

CaMKAR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-AXL

Plasmid

#116714

Purpose

Lentiviral expression of AXL

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AXL (AXL Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-mTagBFP2 PuroR

Plasmid

#101893

Purpose

Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons

Depositor

Ghassan Mouneimne

Insert

Lifeact

Use

Lentiviral

Tags

mTagBFP2

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHACK-GAL4>QF2

Plasmid

#80275

Purpose

GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Expression

Insect

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE60

Plasmid

#90405

Purpose

STAT5 - Stat5 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

STAT5

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB453

Plasmid

#74835

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIB CMV/TO DEST (w320-1)

Plasmid

#17400

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pDONRCBX

Plasmid

#29634

Depositor

David_L Andrews, Scott Gold

Article

Garcia-Pedrajas et al Fungal Genet Biol. 2008 Apr . 45(4):379-

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 19, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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CAG FUCCI

Plasmid

#175266

Purpose

Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

Depositor

James Monaghan

Article

Duerr et al Development. 2022 Mar 15;149(6). pii: 27

Insert

FUCCI

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG Cas9-2A-Citrine

Plasmid

#92358

Purpose

CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9 2A Citrine

Expression

Mammalian

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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ePPE

Plasmid

#183095

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAM5505

Plasmid

#132664

Purpose

pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Insert

MobA

Expression

Bacterial

Mutation

please see depositor comments below

Promoter

p1,p3

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPR-Cas9-ScaligD

Plasmid

#125688

Purpose

Gene knockout/in for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spCas9, sgRNA casette, and ScaligD

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJH124

Plasmid

#102951

Purpose

Expresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoter

Depositor

Peter Espenshade

Article

Hwang et al Biochem J. 2016 Jun 7. pii: BCJ20160106.

Insert

APEX2-NES

Tags

FLAG

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d4EGFP

Plasmid

#41943

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

Plasmid

#113862

Purpose

Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc

Use

Lentiviral

Expression

Mammalian

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-SHP2DA

Plasmid

#12286

Depositor

Anton Bennett

Article

Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

Insert

SHP-2DA (PTPN11 Human)

Tags

Myc

Expression

Mammalian

Mutation

Aspartic Acid 425 mutated to Alanine

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pEF-GalNAc-T2-msGFP2

Plasmid

#140164

Purpose

Expresses the Golgi marker GalnacT2-msGFP2

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

GalNAc-T2-msGFP2 (GALNT2 Human)

Expression

Mammalian

Promoter

EF-1a

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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