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We narrowed to 9,074 results for: nls

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Showing: 4301 - 4320 of 9074 results

  1. pUBC-SV40NLS-Pfv-Sapphire-PURO

    Plasmid
    #203654
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.
    Depositor
    Liam Holt
    Publication
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203654-plasmid-map-sequence-id-402486

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  4. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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  5. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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  6. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    70703-plasmid-map-sequence-id-127765

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  7. pCMV-hA3A-BE3-Y132D

    Plasmid
    #113425
    Purpose
    Expresses hA3A-BE3-Y132D in mammalian cells
    Depositor
    Jia Chen
    Publication
    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hA3A-BE3-Y132D (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    hAPOBEC3A_Y132D
    Promoter
    CMV
    Available since
    Aug. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    113425-plasmid-map-sequence-id-217957

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  8. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

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  9. TV_LGR5-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro

    Plasmid
    #251173
    Purpose
    Targeting vector for C-terminal knockin at LGR5 locus to enable reporting of expression by mNeonGreen and depletion of positive cells with DTR
    Depositor
    Hugo Snippert
    Insert
    ires-mNeon-nls-p2a-dtr-wpre_pgk-puro flanked by homology arms for C-terminal targeting of human LGR5 (LGR5 Human)
    Use
    CRISPR
    Tags
    ires-mNeon-nls-p2a-dtr
    Available since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    251173-plasmid-map-sequence-id-500690

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  14. pTEE

    Plasmid
    #159748
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159748-plasmid-map-sequence-id-313816

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  15. pAAV2-CAG-3xNLS-mScarlet

    Plasmid
    #191098
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Publication
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191098-plasmid-map-sequence-id-380124

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  16. pCR1056

    Plasmid
    #71824
    Purpose
    Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS
    Depositor
    Jacob Corn
    Publication
    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
    Use
    CRISPR
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71824-plasmid-map-sequence-id-131017

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  19. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Publication
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    oDam_f_GFPoNLS
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85819-plasmid-map-sequence-id-170478

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Showing: 4301 - 4320 of 9074 results