We narrowed to 6,247 results for: nls

1. pET-21a-PE2-His

   Plasmid

   #201973

   Purpose

   Express His-tagged PE2 in bacteria

   Depositor

   Scot Wolfe

   Article

   Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

   Insert

   PE2-HIS
   

   Tags

   His and c-myc NLS

   Expression

   Bacterial

   Available Since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP777_SnoopCatcher-p65HSF1-CO

   Plasmid

   #211770

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SnoopCatcher-p65HSF1
   

   Tags

   2xOLLAS

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-nef-CIAO-Flp

   Plasmid

   #149294

   Purpose

   Cre-dependent expression of Flp recombinase.

   Depositor

   Edward Callaway

   Article

   Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

   Insert

   Flp recombinase (flp Budding Yeast)
   

   Use

   AAV

   Mutation

   Derived from the mouse codon-optomized FlpO. The…

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLP775_αGCN4-p65HSF1-CO

   Plasmid

   #211768

   Purpose

   SunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-p65HSF1
   

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ220 (AsCpf1)

   Plasmid

   #86208

   Purpose

   AsCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   AsCpf1
   

   Use

   Gateway compatible cpf1 vectors

   Tags

   5' and 3' NLS

   Expression

   Plant

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-LbCpf1-Blast

   Plasmid

   #84751

   Purpose

   Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   LbCpf1
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-3xHA (C terminal on insert)

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-BE4-PpAPOBEC1 R33A

   Plasmid

   #138345

   Purpose

   Mammalian expression plasmid for BE4-PpABOBEC1 R33A

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-PpAPOBEC1 R33A
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Mutation

   Change of Arg 33 to Ala.

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LLP778_Traptavidin-p65HSF1-CO

   Plasmid

   #211771

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavidin-p65HSF1
   

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LLP776_SpyCatcher-p65HSF1-CO

   Plasmid

   #211769

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SpyCatcher-p65HSF1
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pY011 (pcDNA3.1-hLb2Cpf1)

    Plasmid

    #69983

    Purpose

    Expresses humanized Lb2Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb2Cpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pM125: pAAV-EFS-CasRx-VEGFA array presgRNA

    Plasmid

    #166873

    Purpose

    AAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editing

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Inserts

    U6-VEGFA array presgRNA
    RfxCas13d
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q

    Plasmid

    #132545

    Purpose

    Expresses CIRTS-8 (bdefensin 3-TBP6.7-hADAR(299-701)E488Q) in mammalian cells

    Depositor

    Bryan Dickinson

    Article

    Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

    Insert

    CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q-NLS
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    cmv d0

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP617_αGCN4-mCherry

    Plasmid

    #211785

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SnoopCatcher-DNMT3A
    

    Tags

    2xOLLAS

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LLP783_SpyCatcher-DNMT3A-CO

    Plasmid

    #211776

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavudin-DNMT3A
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. LLP618_αGCN4-KRAB-CO

    Plasmid

    #211782

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-mCherry
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect mCherr…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGAPDH-Cre-HYG

    Plasmid

    #231510

    Purpose

    Expresses Cre recombinase in Acanthamoeba

    Depositor

    Yeonchul Hong

    Insert

    Cre recombinase
    

    Use

    Unspecified; Acanthamoeba castellanii neff strain

    Tags

    EGFP and SV40 NLS

    Promoter

    Acanthamoeba GAPDH

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. (364) SB KRAB-SadCas9

    Plasmid

    #163022

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Promoter

    TET-On

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pY014 (pcDNA3.1-hMbCpf1)

    Plasmid

    #69986

    Purpose

    Expresses humanized MbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hMbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVAX_CBI_AcrIIA4

    Plasmid

    #136662

    Purpose

    Expresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

    Depositor

    Yannick Doyon

    Article

    Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Tags

    No tag, No NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUBC_HA_PP7-mVenus

    Plasmid

    #111268

    Purpose

    Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    PP7-mVenus
    

    Use

    Synthetic Biology

    Tags

    HA, SV40 NLS, and mVenus

    Expression

    Mammalian

    Mutation

    PP7: C68S, S69G [delta]70-75 (non-aggregating)

    Promoter

    hUBC

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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