We narrowed to 11,632 results for: Vars

1. mTFP1-Talin-ABM-10

   Plasmid

   #55510

   Purpose

   Localization: Talin Actin Binding Domain, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   Talin ABM
   

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Actin Binding Module; I/LWEQ = 197 amino acids fr…

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pmalc4x_G252R_TEV

   Plasmid

   #47704

   Purpose

   Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation G252R

   Depositor

   Raquel Lieberman

   Article

   Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

   Insert

   Olfactomedin-like domain with G252R mutation (MYOC Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Glycine 252 Arginine

   Promoter

   Ptac

   Available Since

   Sept. 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pmalc4x_K398R_TEV

   Plasmid

   #47701

   Purpose

   Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation K398R

   Depositor

   Raquel Lieberman

   Article

   Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

   Insert

   Olfactomedin-like domain with K398R mutation (MYOC Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Lysine 398 to Arginine

   Promoter

   Ptac

   Available Since

   Sept. 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAB051e

   Plasmid

   #79195

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PExt-10cons lambda op-62 gIII, luxAB; Ptet lambda cI-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   PExt-10cons lambda op-62; Ptet

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAB078d7

   Plasmid

   #79205

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (off-target) luxAB; Ppro1 434cI-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (off-target); Ppro1

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAB085e

   Plasmid

   #79212

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F7
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ptet

   Availability

   Academic Institutions and Nonprofits only

   

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7. p414GPD-3xFLAG-RBM28 WT

   Plasmid

   #169265

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 WT

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 WT (RBM28 Human)
   

   Tags

   3xFLAG

   Expression

   Yeast

   Promoter

   GPD

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO - RBM28 E8 WT minigene

   Plasmid

   #169271

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 8 WT minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 E8 WT minigene (RBM28 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3/mStayGold(c4)=UtrCH

   Plasmid

   #212020

   Purpose

   For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   UtrCH
   

   Tags

   mStayGold(c4)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-CreERT2 stuffer v3

    Plasmid

    #158046

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pLenti_PE7-2A-BSD

    Plasmid

    #234073

    Purpose

    For lentiviral expression of PE7 with blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PE7
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF-1α core promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-TadA8e-PcrA M6

    Plasmid

    #221229

    Purpose

    Express HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA M6
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVET-IRES-GFP

    Plasmid

    #107139

    Purpose

    A bicistronic lentiviral mammalian expression vector with a multiple cloning site and GFP

    Depositor

    Aki Manninen

    Article

    Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-Cre stuffer v4

    Plasmid

    #158032

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Tags

    mStayGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Expression

    Mammalian

    Mutation

    H840A

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP1830

    Plasmid

    #70708

    Purpose

    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Insert

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    CAG

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE mNeonGreen-Core (HBc from HBV) IRES puro

    Plasmid

    #122202

    Purpose

    Plasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.

    Depositor

    Raphael Gaudin

    Article

    Hallez et al Nat Microbiol. 2019 Apr 1. pii: 10.1038/

    Inserts

    HBc from HBV AYW
    mNeonGreen
    

    Tags

    GGSGGSGGS linker

    Expression

    Mammalian

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFR WT

    Plasmid

    #11011

    Purpose

    Retroviral construct for expressing human EGFR in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4_CRE-CMVmin-luc2

    Plasmid

    #194384

    Purpose

    Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    6xCRE-CMVmin-luc2
    

    Expression

    Mammalian

    Promoter

    CMV minimal

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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