We narrowed to 59,444 results for: cloning

1. MS-1 magA

   Plasmid

   #21751

   Depositor

   Elliot Meyerowitz

   Article

   Bertani et al Gene. 2001 Feb 21. 264(2):257-63.

   Insert

   magA (magA M. magnetotacticum)
   

   Use

   Cloning

   Available Since

   Aug. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pJMP2844

   Plasmid

   #160675

   Purpose

   Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p667-UBC-VRK3-V5-miniTurbo_IDG-K

   Plasmid

   #189902

   Purpose

   Gateway destination clone of VRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   VRK3 (VRK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CTNNB1 in pENTR

   Plasmid

   #216617

   Purpose

   gateway cloning donor vector containing CTNNB1

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Gateway entry plasmid

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EIF5A

   Plasmid

   #25260

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   eIF5A:S15-A151 (EIF5A Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids S15-A151

   Available Since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pLMB509

   Plasmid

   #40084

   Depositor

   Philip Poole

   Article

   Tett et al Appl Environ Microbiol. 2012 Oct;78(19):

   Inserts

   gfpmut 3.1
   tauR
   tau promoter
   

   Expression

   Bacterial

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pV1090

   Plasmid

   #111427

   Purpose

   gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)

   Depositor

   Gerald Fink

   Article

   Vyas et al Sci Adv. 2015;1(3):e1500248.

   Insert

   sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFH114

   Plasmid

   #128408

   Purpose

   Level1 ZmUBIp::BAR:NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   Level1 ZmUBIp::BAR:NOSt (Position 1)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GSK3 alpha pMT2

   Plasmid

   #15896

   Depositor

   Jim Woodgett

   Article

   Woodgett et al EMBO J. 1990 Aug . 9(8):2431-8.

   Insert

   GSK3 alpha (GSK3A Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 1, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. WGA-D

    Plasmid

    #17989

    Depositor

    Natasha Raikhel

    Article

    Smith et al Plant Mol Biol. 1989 Nov . 13(5):601-3.

    Insert

    wheat germ agglutinin isolectin D
    

    Expression

    Bacterial

    Mutation

    N/A

    Available Since

    July 10, 2008

    Availability

    Academic Institutions and Nonprofits only

11. EEF1A2 in pENTR

    Plasmid

    #216585

    Purpose

    gateway cloning donor vector containing EEF1A2

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    EEF1A2 (EEF1A2 Human)
    

    Use

    Gateway entry plasmid

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNCX3.1

    Plasmid

    #67147

    Purpose

    Expresses mRNA of NCX3.1 (Rat brain)

    Depositor

    Michela Ottolia, Kenneth Philipson

    Article

    Nicoll et al J Biol Chem. 1996 Oct 4;271(40):24914-21

    Insert

    NCX3.1
    

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid

    #164637

    Purpose

    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

    Depositor

    Günter Schneider

    Insert

    target guide sequence PRMT5 inhibition (PRMT5 Human)
    

    Use

    CRISPR and Lentiviral; Interference

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DDX3X in pENTR

    Plasmid

    #216572

    Purpose

    gateway cloning donor vector containing DDX3X

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Gateway entry plasmid

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFTC1

    Plasmid

    #64951

    Purpose

    source of TcR FRT cassette

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    FRT-TcR-FRT
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 Osr1

    Plasmid

    #26485

    Depositor

    Paul Danielian

    Article

    So et al Mech Dev. 1999 Jun . 84(1-2):157-60.

    Insert

    Osr1 (Osr1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pFKM1

    Plasmid

    #64950

    Purpose

    source of KmR FRT cassette

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    FRT-KmR-FRT
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6-PspCas13b-non-targeting (pJK5)

    Plasmid

    #229052

    Purpose

    Cloning backbone for PspCas13b crRNA (U6 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221-APN

    Plasmid

    #158440

    Purpose

    Gateway Cloning compatible entry vector for the human APN gene.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    APN (ANPEP Human)
    

    Use

    Gateway entry vector

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p667-UBC-TLK2-V5-TurboID_IDG-K

    Plasmid

    #135250

    Purpose

    Gateway destination clone of TLK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TLK2-V5-TurboID (TLK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge