We narrowed to 8,106 results for: Dos;

1. pEGFP-C1-MKLP1

   Plasmid

   #70145

   Purpose

   mammalian expression of MKLP1 with an eGFP fusion

   Depositor

   Masanori Mishima

   Article

   Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

   Insert

   MKLP1 (KIF23 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FLAG-SOX4

   Plasmid

   #110360

   Purpose

   Mammalian expression of FLAG-tagged SOX4

   Depositor

   Carlos Moreno

   Article

   Liu et al Cancer Res. 2006 Apr 15;66(8):4011-9. do

   Insert

   SOX4 (SOX4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   lacking 5' UTR

   Promoter

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-P2A_BAF_G47E

   Plasmid

   #101774

   Purpose

   Expresses mutant BAF (G47E) in human cells (with EGFP produced from the same transcript as expression control)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP-P2A

   Expression

   Mammalian

   Mutation

   G47E mutation

   Promoter

   EF1a

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Kv7.4 (1-695)/pcDNA3.1

   Plasmid

   #111453

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   8xHis and EGFP

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-695)

   Promoter

   Available since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCIpA102-G-HLA-A2_GFP

   Plasmid

   #85162

   Purpose

   for mRNA expression of HLA.A2-EGFP

   Depositor

   Sébastien Walchli, Tone Fredsvik Gregers

   Article

   Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

   Insert

   HLA.A2 (HLA-A Human)
   

   Use

   Mrna expression

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA-FLAG-METTL3-APPA

   Plasmid

   #160251

   Purpose

   Express catalytic inactive FLAG-METTL3 in human cells

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changes in aa 395–398, DPPW → APPA

   Promoter

   CMV

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDG462

   Plasmid

   #100903

   Purpose

   SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A mutant converts to Nickase

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mini-Ii-Str_SBP-EGFP-LIMP2

   Plasmid

   #222324

   Purpose

   Synchronize the trafficking of LIMP2 from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cav2.2 pcDNA3

   Plasmid

   #206072

   Purpose

   expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

   Depositor

   Annette Dolphin

   Article

   Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pH1R-mCherry-N1

    Plasmid

    #84325

    Purpose

    Histamine-1 receptor tagged with mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H1R (HRH1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EGFP-P2A_BAF

    Plasmid

    #101773

    Purpose

    Expresses BAF in human cells (with EGFP produced from the same transcript as expression control)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-LacI-eGFP

    Plasmid

    #213021

    Purpose

    piggyBac donor vector containing LacI-eGFP for random integration by PBase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    LacI**-eGFP
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-P2A_BAF_L58R

    Plasmid

    #101775

    Purpose

    Expresses mutant BAF (L58R) in human cells (with EGFP produced from the same transcript as expression control)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP-P2A

    Expression

    Mammalian

    Mutation

    L58R mutation

    Promoter

    EF1a

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. HsB2AR-mCherry

    Plasmid

    #137785

    Purpose

    Visualization of the Beta-2-adrenergic receptor

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Human beta2-adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21-pelB-LaG-16

    Plasmid

    #172746

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH3R-mCherry-N1

    Plasmid

    #84327

    Purpose

    Histamine-3 receptor tagged with mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H3R-mCherry (HRH3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICE-FLAG-NAT10-siR-G641E

    Plasmid

    #59366

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Larrieu et al Science. 2014 May 2;344(6183):527-32. do

    Insert

    NAT10 NM_024662 (NAT10 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    G641E and silent mutations to render the cDNA res…

    Promoter

    CMV-tet

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_NR1H2_Hormone-recep

    Plasmid

    #109827

    Purpose

    Protein expression and purification of NR1H2_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR1H2_Hormone-recep (NR1H2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid

    #92140

    Purpose

    Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    AID[71-114]-eGFP
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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