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pDONR223-KDR

Plasmid

#23925

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

KDR (KDR Human)

Use

Gateway donor vector

Mutation

V297I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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AiP12251-pAAV-AiE0453m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2251)

Plasmid

#164453

Purpose

AiE0453m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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NYESO alpha into TRAC HDRT Source (pTR 223)

Plasmid

#112023

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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NYESO beta into TRBC1 HDRT Source (pTR 277)

Plasmid

#112024

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta into TRBC1 HDRT

Use

CRISPR and Synthetic Biology

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-FLT1

Plasmid

#23912

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLT1 (FLT1 Human)

Use

Gateway donor vector

Mutation

S755P

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PAK1

Plasmid

#23543

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK1 (PAK1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DDR1

Plasmid

#23910

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DDR1 (DDR1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-FGR

Plasmid

#23877

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGR (FGR Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
AiP978-pAAV-mscRE4-minBGpromoter-FlpO-WPRE-hGHpA

Plasmid

#163472

Purpose

Direct-expressing FlpO AAV Virus. Alias: AiP978 - pAAV-AiE2004m-minBG-FlpO-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

FlpO

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12014-pAAV-AiE2004m-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN2014)

Plasmid

#164457

Purpose

AiE2004m is an enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minCMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-BUB1B

Plasmid

#23858

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BUB1B (BUB1B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK2

Plasmid

#23658

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK2 (NEK2 Human)

Use

Gateway donor vector

Mutation

Insert encodes truncated protein relative to NP_0…

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CDK7

Plasmid

#23786

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK7 (CDK7 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CALM1

Plasmid

#23768

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CALM1 (CALM1 Human)

Use

Gateway donor vector

Mutation

M74T V144E

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Ai163 (TIT2L-GC6s-ICL-TPT) targeting vector

Plasmid

#114430

Purpose

Target a Cre-dependent GCaMP6s cassette and a tdTomato-P2A-tTA2 cassette to the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GCaMP6s, tdTomato, tTA2

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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AiP1047-pAAV-mscRE16-minBGpromoter-iCre-WPRE-hGHpA

Plasmid

#163479

Purpose

Direct-expressing iCre AAV Virus. Alias: AiP1047 - pAAV-AiE2016m-minBG-iCre-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

iCre

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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AiP12254-pAAV-AiE0459m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2254)

Plasmid

#164454

Purpose

AiE0459m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-MAPK1

Plasmid

#23498

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK1 (MAPK1 Human)

Use

Gateway donor vector

Mutation

missing first codon

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CARD11

Plasmid

#23482

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CARD11 (CARD11 Human)

Use

Gateway donor vector

Mutation

A291C,C292T,1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
AiP12256-pAAV-AiE0462m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2256)

Plasmid

#164456

Purpose

AiE0462m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge