We narrowed to 4,043 results for: golden gate

1. pZA9

   Plasmid

   #158487

   Purpose

   Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Tags

   Expression

   Mutation

   no STOP codon

   Promoter

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZA4

   Plasmid

   #158482

   Purpose

   Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Tags

   Expression

   Mutation

   L17E, D489V, STOP codon

   Promoter

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZA10

   Plasmid

   #158488

   Purpose

   Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1 (ZAR1 Mustard Weed)
   

   Use

   Golden gate entry vector

   Tags

   Expression

   Mutation

   D489V, no STOP codon

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

   Plasmid

   #50626

   Purpose

   pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.

   Depositor

   Andrew Scharenberg

   Type

   Empty backbone

   Use

   Lentiviral and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVL TAL(Nd154C63) T7 Age-Xho/Xba-Sal linkers

   Plasmid

   #50624

   Purpose

   pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.

   Depositor

   Andrew Scharenberg

   Type

   Empty backbone

   Use

   Mrna production

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKT3Ts-goldyTALEN

   Plasmid

   #80330

   Purpose

   Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence

   Depositor

   Jeffrey Essner

   Article

   Welker et al Hum Gene Ther. 2016 Jun;27(6):423-4. doi

   Type

   Empty backbone

   Use

   TALEN

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T3

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNDGG010

   Plasmid

   #231315

   Purpose

   Vector part for BEVA; BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNDGG022

   Plasmid

   #231306

   Purpose

   Vector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNDGG009

   Plasmid

   #231314

   Purpose

   Vector part for BEVA; BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEM-T Easy-Cas9-nls-CDS1

    Plasmid

    #160231

    Purpose

    Cas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    Cas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1
    

    Use

    Level 0 of moclo golden gate position cds1

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZA12

    Plasmid

    #158490

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V/L17E without STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1 (ZAR1 Mustard Weed)
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    L17E, D489V, no STOP codon

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAV10.F6.loxP

    Plasmid

    #63201

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV10.F3.loxP

    Plasmid

    #63200

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAV10.K6.loxP

    Plasmid

    #63203

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV10.HO5.loxP

    Plasmid

    #63206

    Purpose

    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICH47732:FCP:ShBle

    Plasmid

    #85985

    Purpose

    Expresses ShBle with FCP promoter/terminator for F. cylindrus transformation.

    Depositor

    Thomas Mock

    Insert

    ShBle
    

    Use

    Golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    FCP

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXZ13lacZalpha

    Plasmid

    #160229

    Purpose

    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

    Depositor

    Ian Duncan

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. BB1_12_YlpGPD

    Plasmid

    #117820

    Purpose

    Promoter pGPD of Y. lipolytica for GoldenMOCS

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Insert

    YlpGPD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. BB1_23_YlGUT1

    Plasmid

    #117823

    Purpose

    Encodes glycerol kinase (GUT1) of Y. lipolytica for GoldenMOCS

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Insert

    YlGUT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. BB1_34_YlMig1TT

    Plasmid

    #117822

    Purpose

    Transcription terminator Mig1TT of Y. lipolytica for GoldenMOCS

    Depositor

    Michael Sauer

    Article

    Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

    Insert

    YlMig1TT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge