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We narrowed to 2,295 results for: pET28

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pET28b-tglF

Plasmid

#134681

Purpose

Expresses His6-TglF in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglF

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FbGH17B

Plasmid

#100912

Purpose

Expression of the Beta-1,3-Exo-Laminarinase FbGH17B in E.coli

Depositor

Jan-Hendrik Hehemann

Insert

FbGH17Bcat

Use

Tags

His-Tag

Expression

Bacterial

Mutation

not full length

Promoter

Available since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-KinK

Plasmid

#139596

Purpose

expresses KinK in E. coli

Depositor

Emily Balskus

Article

Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

Insert

kinK

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-AlpJ

Plasmid

#139598

Purpose

expresses AlpJ in E. coli

Depositor

Emily Balskus

Article

Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

Insert

alpJ

Use

Tags

N terminal hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-KinO1

Plasmid

#139597

Purpose

expresses KinO1 in E. coli

Depositor

Emily Balskus

Article

Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

Insert

kinO1

Use

Tags

N terminal hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-KinL

Plasmid

#139595

Purpose

expresses KinL in E. coli

Depositor

Emily Balskus

Article

Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

Insert

kinL

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-KinM

Plasmid

#139594

Purpose

expresses KinM in E. coli

Depositor

Emily Balskus

Article

Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

Insert

kinM

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-KinN

Plasmid

#139593

Purpose

expresses KinN in E. coli

Depositor

Emily Balskus

Article

Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

Insert

kinN

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a D315A

Plasmid

#139592

Purpose

expresses SznF D315A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF D315A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed apartate 315 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF

Plasmid

#139564

Purpose

expresses SznF in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-FOXP2_P1

Plasmid

#122645

Purpose

Expresses truncated variant (aa271-aa714) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-FOXP2_P3

Plasmid

#122647

Purpose

Expresses truncated variant (aa367-aa714) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-FOXP2_P4

Plasmid

#122648

Purpose

Expresses truncated variant (aa503-aa714) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3

Plasmid

#124435

Purpose

Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis Tag

Expression

Bacterial

Mutation

Residues 225 to 239 replaced with corresponding s…

Promoter

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-INTS3-C

Plasmid

#128642

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 C-termini (INTS3 Human)

Use

Tags

His tag on N and C-terminal

Expression

Bacterial

Mutation

INTS3 C-termini (514–1042 aa)

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3/S3

Plasmid

#124627

Purpose

Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 237 to 241 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28B-PU.1-ETS-S3

Plasmid

#124550

Purpose

Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 242 to 246 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-PU.1-ETS-H2

Plasmid

#123358

Purpose

Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 207 to 215 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-YJR129c

Plasmid

#85118

Purpose

bacterial expression of YJR129c

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFM3 (EFM3 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(WT)

Plasmid

#112720

Purpose

Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(WT)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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