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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 839 results for: pFAST

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Showing: 421 - 440 of 839 results

  1. pFB-LIC-Bse

    Plasmid
    #26108
    Purpose
    SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Opher Gileadi
    Publication
    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    His6 - TEV
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    Oct. 4, 2010
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    26108-plasmid-map-sequence-id-12260

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  4. pCoofy44

    Plasmid
    #55185
    Purpose
    Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Publication
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…
    Expression
    Bacterial
    Promoter
    pPH (Polyhedrin promoter)
    Available since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55185-plasmid-map-sequence-id-85559

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  5. pDR121

    Plasmid
    #37148
    Depositor
    Dale Ramsden
    Insert
    XRCC6 (XRCC6 Human)
    Tags
    H6
    Expression
    Insect
    Available since
    July 18, 2012
    Availability
    Academic Institutions and Nonprofits only

  6. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

    Plasmid
    #86843
    Purpose
    HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.
    Depositor
    Thomas Cech
    Publication
    Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d
    Insert
    FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
    Tags
    FLAG-HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous TERT promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    86843-plasmid-map-sequence-id-174395

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  8. pDR123

    Plasmid
    #37149
    Depositor
    Dale Ramsden
    Insert
    XRCC5 (XRCC5 Human)
    Expression
    Insect
    Available since
    July 18, 2012
    Availability
    Academic Institutions and Nonprofits only

  9. pSR651

    Plasmid
    #65213
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Publication
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-1 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    p10 and polyhedrin
    Available since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65213-plasmid-map-sequence-id-112889

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  11. pDR125

    Plasmid
    #37150
    Depositor
    Dale Ramsden
    Insert
    lig4 (LIG4 Human)
    Tags
    H6
    Expression
    Insect
    Available since
    July 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  14. pCoofy44

    Plasmid
    #55185
    Purpose
    Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Publication
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…
    Expression
    Bacterial
    Promoter
    pPH (Polyhedrin promoter)
    Available since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55185-plasmid-map-sequence-id-85559

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  15. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

    Plasmid
    #86843
    Purpose
    HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.
    Depositor
    Thomas Cech
    Publication
    Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d
    Insert
    FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
    Tags
    FLAG-HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous TERT promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    86843-plasmid-map-sequence-id-174395

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Showing: 421 - 440 of 839 results