We narrowed to 4,687 results for: puc19

1. p1977 (pUC19-PTDH3-PTEF1)

   Plasmid

   #78230

   Purpose

   Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   Bidirectional promoters pTDH3-pTEF1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pUC19.TRIM28.FKBP.knock-in.mCerulean

   Plasmid

   #185009

   Purpose

   For knocking in FKBP F36V P2A mCerulean

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   FKBP F36V P2A mCerulean
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-link-PAmCherry-FLAG:cat

   Plasmid

   #211832

   Purpose

   Plasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.

   Depositor

   Peng Chen

   Insert

   PAmCherry-FLAG-cat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19, containing H2-Kd Balb/c WK10

   Plasmid

   #102642

   Purpose

   Encodes sequence of the murine H2-Kd allele

   Depositor

   Sai Reddy

   Article

   Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

   Insert

   H2-Kd Balb/c
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-link-PAGFP-FLAG:Kan

   Plasmid

   #211834

   Purpose

   Plasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.

   Depositor

   Peng Chen

   Insert

   PAGFP-FLAG-Kan
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-TSV (linearized in VP2)

   Plasmid

   #70032

   Purpose

   pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region

   Depositor

   Mariet Feltkamp

   Article

   van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

   Insert

   TSV full genome, linearized in VP2 gene
   

   Use

   Cloning vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB1975 (pUC19-PTEF1-PPGK1)

   Plasmid

   #73296

   Purpose

   Example plasmid containing a bidirectional promoter

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   pTEF1-pPGK1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-Top-Toehold-mCherry

   Plasmid

   #87318

   Purpose

   Expresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19 ACMV DNA-B 1.5mer

   Plasmid

   #159135

   Purpose

   Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-B

   Depositor

   Vincent Fondong

   Article

   Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

   Insert

   ACMV DNA-B 1.5mer
   

   Use

   Infectious clone for bombarding plants

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-P70a-Top-T~Bottom-T

    Plasmid

    #87315

    Purpose

    Control plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system (promoter control)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19 gene 60 delta gap

    Plasmid

    #49091

    Purpose

    Template for generating gene 60 mRNA, coding gap deletion

    Depositor

    Nils Walter

    Article

    Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

    Insert

    Bacteriophage T4 gene 60
    

    Use

    Tags

    Expression

    Mutation

    Coding gap and coding gap STOP have been deleted.

    Promoter

    T7

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mishler2014-No-aptamer-controlluciferase-pUC19

    Plasmid

    #63856

    Purpose

    This pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.

    Depositor

    Howard Salis

    Article

    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

    Insert

    No-aptamer control mRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    tac

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pUC19::ZmUBI-RWT4Ca_K217R/K327R

    Plasmid

    #237511

    Purpose

    Protoplast expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    Rwt4Ca_K217R/K327R
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutations at kinase active sites (K217R, K327R)

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19_msfGFP-HP1a-repair-template

    Plasmid

    #238248

    Purpose

    For knock-in msfGFP to HP1a locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    HP1a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19_msfGFP-TCOF1-repair-template

    Plasmid

    #237632

    Purpose

    For knock-in msfGFP to TCOF1 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    TCOF1 (TCOF1 Human)
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19_msfGFP-FUS-repair-tempate

    Plasmid

    #237684

    Purpose

    For knock-in msfGFP to FUS locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    FUS (FUS Human)
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19::ZmUBI-RWT4Ca+C-Tail

    Plasmid

    #237514

    Purpose

    Protoplast expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    Rwt4Ca+C-Tail
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Add C-terminal extension

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19-U6-Bs3-sgRNA (BsaI)

    Plasmid

    #188280

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

    Insert

    Bs3-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    U6

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19 KGA HA5'-mKO2-3'

    Plasmid

    #110406

    Purpose

    Homology donor for glutaminase KGA isoform knock-in, mKO2

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. puC19 preACT1(del6) short BP-3'SS

    Plasmid

    #111333

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    shorter branch to 3' splice site distance

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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