We narrowed to 15,368 results for: NOL

1. pBAD-HisD-miniGFP1

   Plasmid

   #188896

   Purpose

   Express 6xHis-Tag miniGFP1 in E.coli

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   miniGFP1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRC0940 (circRNA entry vector)

   Plasmid

   #189967

   Purpose

   Cloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestion

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHT-ads

   Plasmid

   #47382

   Purpose

   Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pgrac

   Available since

   Oct. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac-M2

   Plasmid

   #135583

   Purpose

   High eukaryotic protein expression shuttle vector

   Depositor

   Xiao-Wen Cheng

   Article

   Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

   Type

   Empty backbone

   Use

   Transfer vector

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin of AcMNPV

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_ABEmax_P2A_GFP

   Plasmid

   #112101

   Purpose

   A:T-to-G:C base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   ABEmax_P2A_GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHT-trxA.ads

   Plasmid

   #47383

   Purpose

   Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Use

   Tags

   6xHis and trxA

   Expression

   Bacterial

   Mutation

   Promoter

   Pgrac

   Available since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBZS-M.CviPI

   Plasmid

   #198355

   Purpose

   Express M.CviPI in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   M.CviPI (M.CviPI )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AbVec2.0-IGHG1

   Plasmid

   #80795

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti6.2_mNeonGreen2

   Plasmid

   #113727

   Purpose

   Expresses the fluorescent protein mNeonGreen2 in a lentiviral backbone

   Depositor

   Vanessa LaPointe

   Article

   Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

   Insert

   mNeonGreen2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AbVec1.1-IGKC

    Plasmid

    #80796

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. gCH128 (non-targeting crRNA)

    Plasmid

    #217339

    Purpose

    Non-targeting crRNA

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    crNT-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-10His-mStayGold (E138D)

    Plasmid

    #211363

    Purpose

    Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Mammalian

    Mutation

    E138D

    Promoter

    CMV

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. NM9-SpCas9-NLS3

    Plasmid

    #128177

    Purpose

    Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    SpCas9-NLS3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDAC439

    Plasmid

    #195237

    Purpose

    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRC0569 (parts entry vector)

    Plasmid

    #189966

    Purpose

    Cloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    BBa-J23100

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. tdTomato-pBAD

    Plasmid

    #54856

    Purpose

    Localization: Protein Expression Vector , Excitation: 554, Emission: 581

    Depositor

    Nathan Shaner, Michael Davidson, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Type

    Empty backbone

    Use

    Tags

    6xHis and tdTomato

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV_BE4max

    Plasmid

    #112093

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. gag-P3-pol

    Plasmid

    #211374

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-P3-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-tmpknot-GG-acceptor

    Plasmid

    #174039

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-MBP-mSA2

    Plasmid

    #52319

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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