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Addgene

We narrowed to 5,907 results for: SUP

Showing: 4381 - 4400 of 5907 results
  1. A1-LCD allW D262V

    Plasmid
    #234616
    Purpose
    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
    Depositor
    Insert
    hnRNPA1_LCD_allW D262V (HNRNPA1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    C-terminal domain (186-320) bearing familial ALS …
    Promoter
    T7
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. A1-LCD allW D262N

    Plasmid
    #234615
    Purpose
    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
    Depositor
    Insert
    hnRNPA1_LCD_allW D262N (HNRNPA1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    C-terminal domain (186-320) bearing familial ALS …
    Promoter
    T7
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pET-hMICU1EF

    Plasmid
    #199180
    Purpose
    This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.
    Depositor
    Insert
    MICU1 (MICU1 Human)
    Use
    Tags
    His6 and MBP, Thrombin site, TEV site
    Expression
    Mutation
    D421A, E432K, F433W, F453W
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pDEST-Myc-FBW7]K404R

    Plasmid
    #200715
    Purpose
    The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for overexpression and detection by western blotting.
    Depositor
    Insert
    FBW7 (FBXW7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW7 K404 is mutated to Alanine.
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pDEST-Myc-FBW7K404/412/R

    Plasmid
    #200716
    Purpose
    The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 & 412 to Arg mutation. Used for mammalian overexpression and detection by western blotting.
    Depositor
    Insert
    FBW7 (FBXW7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW7 K404 and K412 mutated to Alanines.
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 4381 - 4400 of 5907 results