Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 13,230 results for: CRISPR-Cas9

Showing: 4421 - 4440 of 13230 results

  5. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA Ser promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166700-plasmid-map-sequence-id-329224

    Enlarge

  6. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-474294

    Enlarge

  7. Adeno EA

    Plasmid
    #64071
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Insert
    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Expression
    Mutation
    Promoter
    U6 and CBh
    Available since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pLSB-NAT

    Plasmid
    #166698
    Purpose
    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166698-plasmid-map-sequence-id-329407

    Enlarge

  10. pX458-DsRed2

    Plasmid
    #112219
    Purpose
    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2
    Depositor
    Yinming Liang
    Article
    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:
    Insert
    DsRed2
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    DsRed2 contains a silent nucleotide substitution …
    Promoter
    Available since
    July 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    112219-plasmid-map-sequence-id-215205

    Enlarge

  11. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

    Enlarge

  12. pUDP012

    Plasmid
    #101167
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101167-plasmid-map-sequence-id-199268

    Enlarge

  13. pUDP044

    Plasmid
    #101168
    Purpose
    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101168-plasmid-map-sequence-id-199309

    Enlarge

  18. pXZ13lacZalpha

    Plasmid
    #160229
    Purpose
    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160229-plasmid-map-sequence-id-314764

    Enlarge

Showing: 4421 - 4440 of 13230 results