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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,962 results for: pcDNA3.1+

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Showing: 4441 - 4460 of 6962 results

  1. cyt-GafD-mTurboID-V5

    Plasmid
    #184640
    Purpose
    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Publication
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cyt-GlycoID-V5
    Tags
    Nuclear export sequence and V5 tag
    Expression
    Mammalian
    Mutation
    GafD -> expressed aa22-178 ; BirA* -> expre…
    Promoter
    CMV
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184640-plasmid-map-sequence-id-366751

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  2. pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc

    Plasmid
    #161585
    Purpose
    expression of Myc-tagged mouse Lrrk2 containing Parkinson's associated G2019S mutation
    Depositor
    Maik Hintze
    Insert
    Lrrk2 (Lrrk2 Mouse)
    Tags
    Myc tag and StrepII
    Expression
    Mammalian
    Mutation
    G2019S
    Promoter
    CMV
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    161585-plasmid-map-sequence-id-318589

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  6. pcDNA3-sPDGFRalpha-Fc

    Plasmid
    #136438
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    PDGFRalpha (PDGFRA Human)
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524)
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136438-plasmid-map-sequence-id-271697

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  8. pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI

    Plasmid
    #126480
    Purpose
    Expresses Cas9-GSAlinker-TdT in mammalian cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    GSA linker
    TdT (DNTT Human)
    Expression
    Mammalian
    Mutation
    Changed codons to reduce repetitive sequences.
    Promoter
    CMV
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126480-plasmid-map-sequence-id-246056

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  10. myc-BioID-Rab7

    Plasmid
    #121049
    Purpose
    Expresses myc tagged BioID and human Rab7a in mammalian cells
    Depositor
    Gia Voeltz
    Publication
    Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    BioID
    Rab7A
    Tags
    myc
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    CMV
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121049-plasmid-map-sequence-id-235292

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  11. eGFP-proα2(I)-G610C

    Plasmid
    #119827
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Publication
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    119827-plasmid-map-sequence-id-233848

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  12. VinculinTS-A50I

    Plasmid
    #111827
    Purpose
    Vinculin tension sensor with point mutation affecting ability to bind talin
    Depositor
    Brenton Hoffman
    Publication
    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VinculinTS (A50I)
    Expression
    Mammalian
    Mutation
    changed alanine 50 to isoleucine
    Promoter
    CMV
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111827-plasmid-map-sequence-id-215129

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  18. pcDNA3-optoB2-SS-YFP

    Plasmid
    #75270
    Purpose
    Arrestin independent version of optoB2 with serine mutations at S362A,S363G
    Depositor
    Michael Bruchas
    Publication
    Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Arrestin independent version of optoB2
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    serine mutations at S362A,S363G
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75270-plasmid-map-sequence-id-149684

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  19. InsRA-EBFP2

    Plasmid
    #79797
    Purpose
    Insulin receptor isoform A with a inter-domain EBFP2 tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
    Depositor
    James Johnson
    Publication
    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    InsRA (INSR Human)
    Tags
    EBFP2 extracellular
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 5, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79797-plasmid-map-sequence-id-153614

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Showing: 4441 - 4460 of 6962 results