We narrowed to 32,527 results for: Eng;
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Plasmid#188314PurposeExpression of eGFP-Luz via a T7 promoter in TXTLDepositorInsertGFP-Luz fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialPromoterT7MaxAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuxE
Plasmid#188309PurposeExpression of LuxE via a T7 promoter in TXTLDepositorInsertluxE
UseSynthetic BiologyExpressionBacterialPromoterT7MaxAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
p09008_AF1-4+GFP5-7
Plasmid#173715PurposePlasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.DepositorInsertshybrid GFP
mKate2
UseE. coli cell free protein synthesis extractsExpressionBacterialAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_E2At(2nd)
Plasmid#183085Purpose2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertE2A
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_O2At(2nd)
Plasmid#183086Purpose2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertO2A
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_P2At(2nd)
Plasmid#183087Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertP2A
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-amylase sensor
Plasmid#186293PurposeExpression alpha-amylase sensor in e coliDepositorInsertcpLumiLuc-amylase sensor
TagsHisTagExpressionBacterialAvailable SinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-HSA sensor
Plasmid#186294PurposeExpression Human serum albumin sensor in e coliDepositorInsertcpLumiLuc-HSA sensor
TagsHisTagExpressionBacterialAvailable SinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterTag-3xL-Δtag
Plasmid#186914PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, expressing sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side BFP and puromycin resistance geneDepositorInsertsfCherry11-(GGGGS)3-PDGFR
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterTag-2xL
Plasmid#186912PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)2 linker and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-(GGGGS)2-PDGFR
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-2xL
Plasmid#186908PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)2 linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)2-SpyCatcher003-sfCherry1-10
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch
Plasmid#186906PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-SpyCatcher003-sfCherry1-10
ExpressionMammalianAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-sfGFP
Plasmid#180756PurposepET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
Tagsstrep tagExpressionBacterialAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK035
Plasmid#180827PurposeT7-inducible expression construct to produce SwGdmB (HY78_09235) in E. coliDepositorArticleInsertSwGdmB
Tags6X HisExpressionBacterialPromoterT7Available SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK036
Plasmid#180828PurposeT7-inducible expression construct to produce CnGdmA (CNE_RS35785) in E. coliDepositorArticleInsertCnGdmA
Tags6X HisExpressionBacterialPromoterT7Available SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSB-FICAT_Payload-Esp3I
Plasmid#179380PurposeTransposon vector prepared for Esp3I golden gate cloning of TransgeneDepositorTypeEmpty backboneExpressionMammalianAvailable SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xalb_I-F1_array_GG
Plasmid#178770PurposeGolden Gate vector to generate X. albilineans type I-F1 single arraysDepositorInsertGolden Gate vector BaeI recognition site to generate X. albilineans type I-F1 single arrays
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sth_I-E array_GFP dropout
Plasmid#178775PurposeGolden Gate GFP dropout vector to generate S. thermophilus type I-E single arraysDepositorInsertgfp dropout to generate S. thermophilus type I-E single arrays
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEh_gRNA1
Plasmid#178758PurposeExpression of a gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E systemDepositorInsertgRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMs_gRNA1
Plasmid#178764PurposeExpression of a gRNA that targets next to PAM library, used for Marinomonas sp. type I-E systemDepositorInsertgRNA that targets next to PAM library, used for Marinomonas sp. type I-E system
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xalb_I-C_array_GG
Plasmid#178767PurposeGolden Gate vector to generate X. albilineans type I-C single arraysDepositorInsertGolden Gate vector BaeI recognition site to generate X. albilineans type I-C single arrays
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc3_gRNA1
Plasmid#178746PurposeExpression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 systemDepositorInsertgRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLm_gRNA1
Plasmid#178752PurposeExpression of a gRNA that targets next to PAM library, used for L. mobilis type I-E systemDepositorInserta gRNA that targets next to PAM library, used for L. mobilis type I-E system
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc2_gRNA1
Plasmid#178740PurposeExpression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 systemDepositorInsertgRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3137
Plasmid#179617PurposePunc-4::GCaMP6::RFP unc-54 3' UTR C.elegans A-MNs and others expression of GCaMP6::RFPDepositorInsertGCaMP6
TagsRFPExpressionWormPromoterPunc-4Available SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAAAG
Plasmid#170096PurposedeGFP reporter plasmid with CAAAG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAATG
Plasmid#170097PurposedeGFP reporter plasmid with CAATG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTAAT
Plasmid#170098PurposedeGFP reporter plasmid with GTAAT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTATT
Plasmid#170099PurposedeGFP reporter plasmid with GTATT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PacI
Plasmid#170100PurposedeGFP reporter plasmid with unique PacI restriction enzyme recognition siteDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEc-crRNA2
Plasmid#170089Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyPromoterJ23119Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas5
Plasmid#170090Purposeexpresses E. coli type I-E Cas5DepositorInsertE. coli type I-E Cas5
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas7
Plasmid#170092Purposeexpresses E. coli type I-E Cas7DepositorInsertE. coli type I-E Cas7
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas8
Plasmid#170093Purposeexpresses E. coli type I-E Cas8DepositorInsertE. coli type I-E Cas8
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCse2
Plasmid#170094Purposeexpresses E. coli type I-E Cse2DepositorInsertE. coli type I-E Cse2
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATAAC
Plasmid#170095PurposedeGFP reporter plasmid with ATAAC PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA43
Plasmid#172944PurposepQE30 encoding Wzz mutant i191DepositorInsertShigella flexneri wzz i191 mutant gene
Tags6xHisExpressionBacterialAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzBST
Plasmid#172968PurposepWSK29 with Salmonella typhimurium wzzBST geneDepositorInsertwzzB gene from Salmonella enterica serovar Typhimurium
ExpressionBacterialMutationK198E- please see depositor commentsAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA47
Plasmid#172947PurposepQE30 encoding Wzz mutant i255DepositorInsertShigella flexneri wzz i255 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-fepEST
Plasmid#172969PurposepWSK29 with Salmonella typhimurium fepEST geneDepositorInsertfepE gene from Salmonella enterica serovar Typhimurium
ExpressionBacterialMutationG160S- please see depositor commentsAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA49
Plasmid#172948PurposepQE30 encoding Wzz mutant i81DepositorInsertShigella flexneri wzz i81 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA42
Plasmid#172943PurposepQE30 encoding Wzz mutant i290DepositorInsertShigella flexneri wzz i290 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA52
Plasmid#172938PurposepQE30 encoding Wzz mutant i138DepositorInsertShigella flexneri wzz i138 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA41
Plasmid#172935PurposepQE30 encoding Wzz mutant i231DepositorInsertShigella flexneri wzz i231 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA56
Plasmid#172951PurposepQE30 encoding Wzz mutant i66DepositorInsertShigella flexneri wzz i66 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA46
Plasmid#172946PurposepQE30 encoding Wzz mutant i279DepositorInsertShigella flexneri wzz i279 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA51
Plasmid#172949PurposepQE30 encoding Wzz mutant i247DepositorInsertShigella flexneri wzz i247 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA40
Plasmid#172942PurposepQE30 encoding Wzz mutant i163DepositorInsertShigella flexneri wzz i163 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA39
Plasmid#172940PurposepQE30 encoding Wzz mutant i128DepositorInsertShigella flexneri wzz i128 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA44
Plasmid#172945PurposepQE30 encoding Wzz mutant i161DepositorInsertShigella flexneri wzz i161 mutant gene
Tags6xHisExpressionBacterialAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only