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pBBK27 pHomL-RPL18Bp-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179011

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-SpEdc1opt_155-180-GB1_AD

Plasmid

#148544

Purpose

Bacterial Expression of SpEDC1opt_155-180

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpEDC1opt_155-180

Expression

Bacterial

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HCV-Fluc_AC

Plasmid

#148447

Purpose

Mammalian Expression of Fluc

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

Fluc

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-Rluc-6xbsMS2-random_AC

Plasmid

#148431

Purpose

Mammalian Expression of 6xbsMS2-random

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-random

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C_1248-1690-delMID_I

Plasmid

#146593

Purpose

Mammalian Expression of HsTNRC6C_1248-1690-delMID

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6C_1248-1690-delMID (TNRC6C Human)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1_C

Plasmid

#146087

Purpose

Mammalian Expression of HsTNRC6Ciso1

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6Ciso1 (TNRC6C Human)

Expression

Mammalian

Mutation

two silent and three non silent mutations, S337G,…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C_C

Plasmid

#146089

Purpose

Mammalian Expression of HsTNRC6C

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C (TNRC6C Human)

Expression

Mammalian

Mutation

3 silent mutations compared to the sequence of Hs…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJH76

Plasmid

#172144

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.2TAG, luxAB; Plpp pylS; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*, Y184*)

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pJH578

Plasmid

#172162

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2_v2); AraC

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH767

Plasmid

#172172

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WT]

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB]

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH857

Plasmid

#172175

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WTopt]

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH202

Plasmid

#172150

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp 32A; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*); 32A pylS variant

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH223

Plasmid

#172151

Purpose

pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/lac (pylBCD 34B_sub-pop5); LacI

Expression

Bacterial

Mutation

PylB(N61S, E178K, E251D) PylD(A252V)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH224

Plasmid

#172152

Purpose

pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/lac (pylBCD 40C_sub-pop6); LacI

Expression

Bacterial

Mutation

PylB(N61S, E175K, E178K)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH463

Plasmid

#172155

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 36A_sub-pop1); AraC

Expression

Bacterial

Mutation

PylB(N61S, E122K, E178K, G188R)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH573

Plasmid

#172157

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (Untagged pylBCD); AraC

Expression

Bacterial

Mutation

Codon-optimized Methanosarcina acetivorans pylBCD

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH574

Plasmid

#172158

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 36A_sub-pop2); AraC

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K) PylC(E316D)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH575

Plasmid

#172159

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 34B_sub-pop3); AraC

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E178K, G188R, E251D) PylD(A2…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH576

Plasmid

#172160

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 34B_sub-pop4); AraC

Expression

Bacterial

Mutation

PylB(N61S, E175K, E178K, E251D) PylD(A252V)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH60

Plasmid

#172142

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.CD

Expression

Bacterial

Mutation

PylCD partial operon (PylB deleted to assess impa…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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