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CRISPRi sgPTPN11-1

Plasmid

#253090

Purpose

CRISPRi single-guide against PTPN11

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

PTPN11 (PTPN11 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mouse Slc9c1 (ΔExchanger)-PA

Plasmid

#249414

Purpose

Expression vector of mouse Slc9c1(aa588-1175) tagged with PA at C-terminus under the CAG promoter

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Slc9c1 (Slc9c1 Mouse)

Tags

PA

Expression

Mammalian

Mutation

deleted amino acids 1-587

Promoter

CAG

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mouseSlc9c1(ΔExchanger/ΔVSD (S1-S3))-PA

Plasmid

#249413

Purpose

Expression vector of mouse Slc9c1(aa733-1175) tagged with PA at C-terminus under the CAG promoter

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Slc9c1 (Slc9c1 Mouse)

Tags

PA

Expression

Mammalian

Mutation

deleted amino acids 1-732

Promoter

CAG

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mouse Slc9c1 (C-term)-PA

Plasmid

#249415

Purpose

Expression vector of mouse SLC9C1 (aa1026-1175) tagged with PA at C-terminus under the CAG promoter.

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Slc9c1 (Slc9c1 Mouse)

Tags

PA

Expression

Mammalian

Mutation

deleted amino acids 1-1025

Promoter

CAG

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pTW368z

Plasmid

#246581

Purpose

Golden gate acceptor for AtRaf1 library cloning

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

sfGFP, AtRaf2, AtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (RbcX2, CPN60B, CPN20, CPN60A Mustard Weed)

Expression

Bacterial

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTW334e3

Plasmid

#246588

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN20, CPN60B, CPN60A Mustard Weed)

Expression

Bacterial

Available Since

Nov. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411c

Plasmid

#246584

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, NtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411a

Plasmid

#246582

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN20, CPN60A, AT3G04550 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-Fbxw7opt

Plasmid

#241795

Purpose

Expresses Fbxw7 gene in mammalian cells.

Depositor

Diego Castrillon

Article

Cuevas et al Proc Natl Acad Sci U S A. 2019 Nov 26. p

Insert

Fbxw7 (FBXW7 Human)

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-2pabPAC

Plasmid

#234547

Purpose

to express the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes

Depositor

Hajime Hirase

Article

Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

2pabPAC

Use

AAV

Mutation

None

Promoter

mGFAP(ABC1D)

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD(276-399_C299S_F362A_S390C)

Plasmid

#188048

Purpose

Expresses His8-FLI1_DBD(276-399_C299S_F362A_S390C) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 400-452, mutated C299S…

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD_R2L2

Plasmid

#188049

Purpose

Expresses His8-FLI1_DBD_R2L2(276-399_R337L_R340L_F362A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 400-452, mutated R337L…

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD_deltaAlpha4

Plasmid

#188050

Purpose

Expresses His8-FLI1_DBD_deltaAlpha4(276-361) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 362-452

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-ERG_DBD(306-429_F392A)

Plasmid

#188051

Purpose

Expresses His8-ERG_DBD(306-429_F392A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

ERG (ERG Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-305, 430-479, mutated F392A

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD(276-399_F362A)

Plasmid

#188047

Purpose

Expresses His8-FLI1_DBD(276-399_F362A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 400-452, mutated F362A

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Endo-InPGrp

Plasmid

#236003

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to endosomes.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Endo-InPGrp (Cyth3 Rat, Synthetic)

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger), 6xHIS -…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pM156: pAAV.CMV-Cas13e (GenScript CO)-VEGFA sgRNA

Plasmid

#203452

Purpose

Plasmid expressing active and codon optimised Cas13e with VEGFA gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ILF3-ts1

Plasmid

#174265

Purpose

ILF3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ILF3 shRNA (ILF3 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ILF3-ts2

Plasmid

#174266

Purpose

ILF3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ILF3 shRNA (ILF3 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ILF3-ts3

Plasmid

#174267

Purpose

ILF3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ILF3 shRNA (ILF3 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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