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pBS cGli3 (CT#177)

Plasmid

#13954

Depositor

Cliff Tabin

Article

Schweitzer et al Mech Dev. 2000 Nov . 98(1-2):171-4.

Insert

Gli3 (GLI3 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
DD-N-nLuc-QtSig

Plasmid

#236777

Purpose

Expresses the luminescent protein nLuc fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

DD-N-nLuc-QtSig

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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HA_DD-N-mScarlet-I-QtSig

Plasmid

#236775

Purpose

Expresses the red fluorescent protein mScarlet-I fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

HA_DD-N_mScarlet-I_QtSig

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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HA_DD-N-mTagBFP2-QtSig

Plasmid

#236776

Purpose

Expresses the blue fluorescent protein mTagBFP2 fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

DD-N, TagBFP, QtSig

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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EML6428_ pRL662-eGFP

Plasmid

#234350

Purpose

Expressed eGFP(S65T) in agrobacteria for tracing in planta

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

SpeR

Expression

Bacterial

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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EML6442_p35S-mCherry2-SV40

Plasmid

#234351

Purpose

Expressed mCherry2 florescent proteins in plant cells for tracing transformation events

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

SpeR

Expression

Plant

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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EML8357_pA3A-CBECas9

Plasmid

#234352

Purpose

A base editor derived by egg-cell specific promoter

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pSC-MRscRNA

Plasmid

#240211

Purpose

Yeast vector expressing theophylline-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

Theophylline MR-scRNA targeting TetO

Expression

Yeast

Promoter

SNR52

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHS-dCas9

Plasmid

#240212

Purpose

Mammalian vector for transient expression of dCas9

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

dCas9

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHS-MCP-VPR

Plasmid

#240213

Purpose

Mammalian vector for transient expression of MCP-VPR

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

MCP-VPR

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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CAG-iRFP

Plasmid

#140460

Purpose

Encodes iRFP driven by the CAG promoter

Depositor

Mark Emerson

Article

Buenaventura et al Dev Biol. 2018 Nov 1;443(1):35-49. doi:

Inserts

CAG promoter
iRFP670

Use

Chicken expression

Expression

Mammalian

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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P-fusA2

Plasmid

#235656

Purpose

Reports Cur activity in Bacteroides thetaiotaomicron

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides thetaiotaomicron fusA2 promoter

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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Halo XLF sgRNA

Plasmid

#207605

Purpose

sgRNA for the insert of the HaloTag at the endogenous loci of XLF

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

GTTCTTCCATctgcaaaaaa

Tags

None

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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HaloXRCC4 sgRNA

Plasmid

#207606

Purpose

sgRNA for the insert of the HaloTag at the endogenous loci of XRCC4.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

TACTGGGTTCAGAAACAAGG

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Med15

Plasmid

#231887

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Med15

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Med15

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pZZMP132

Plasmid

#231757

Purpose

Mammalian expression of SPO11-EA

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11-EA (Spo11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

E224A

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pRDB_268

Plasmid

#231112

Purpose

SpG-Cas9

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

Cas9-SpG

Use

CRISPR and Lentiviral

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA566

Plasmid

#231115

Purpose

Fragmid fragment: (guide cassette) ABE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA568

Plasmid

#231117

Purpose

Fragmid fragment: (guide cassette) CBE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAM198

Plasmid

#227657

Purpose

pRS305 His-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

His-HRV3C-

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM200

Plasmid

#227659

Purpose

pRS305 3XFLAG-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

3X FLAG-HRV3C-

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM202

Plasmid

#227661

Purpose

pRS316 Rpn6 promoter-Rpn5 ORF-Rpn5 terminator

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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JD134

Plasmid

#229490

Purpose

Adenoviral E4 gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E4

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

wt promoter

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCB-CRISPRi

Plasmid

#221136

Purpose

Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

cbu1169 promoter

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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GFP H2B SL2

Plasmid

#227718

Purpose

Plasmid contains GFP H2B SL2, with some nlp-51 homology sequence after, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

GFP H2B SL2 (nlp-51 Nematode)

Use

Cloning vector

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM034_P7_3"Lys3

Plasmid

#216459

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM038_P8b_5'Lys3

Plasmid

#216463

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHA8

Plasmid

#223650

Purpose

BccP protein purification

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

BccP

Tags

MBP

Expression

Bacterial

Promoter

LacI

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA11

Plasmid

#223643

Purpose

Expression of ipgC-turboID fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

ipgC (ipgC )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA12

Plasmid

#223644

Purpose

Expression of turboID-ipgC fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

ipgC (ipgC )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge