We narrowed to 11,215 results for: Kars

1. Intron-Tagging-mScarlet-Frame1-Parental-Minicircle

   Plasmid

   #216125

   Purpose

   Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   sgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV-EF1A-LivePAR-Hygro

   Plasmid

   #176063

   Purpose

   EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   LivePAR
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PM-RspA-CFAST

   Plasmid

   #233601

   Purpose

   Expression of RspA-CFAST on the PM facing the cytosol

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   RspA-CFAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFUW-TetO-ZFP36

   Plasmid

   #178438

   Purpose

   doxycycline-inducible expression of Human ZFP36 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   ZFP36 (ZFP36 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TetO-FUW-Lhx2

   Plasmid

   #61537

   Purpose

   Expresses Lhx2 in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   Lhx2 (Lhx2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PPIB

   Plasmid

   #155838

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PPIB (PPIB Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTet-O-NEUROD1-T2A-PuroR

   Plasmid

   #162338

   Purpose

   Lentiviral expression of NEUROD1 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   NEUROD1 (NEUROD1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Intron-Tagging-EGFP-Frame0-Parental-Minicircle

   Plasmid

   #216124

   Purpose

   Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBMN mEGFP-OPTN

   Plasmid

   #188784

   Purpose

   For mammalian expression of OPTN tagged with mEGFP

   Depositor

   Michael Lazarou

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   OPTN (OPTN Human)
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

    Plasmid

    #203229

    Purpose

    Cre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

    Depositor

    Adam Cohen

    Insert

    Cre-dependent, membrane-localized Optopatch
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET14b His Rab8a Q67L

    Plasmid

    #186014

    Purpose

    His Rab8a Q67L

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    His Rab8a Q67L (RAB8A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRB133

    Plasmid

    #180176

    Purpose

    GluN2A in pCDNA3.1

    Depositor

    Richard Bergeron

    Article

    Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

    Insert

    GluN2A (Grin2a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3 Flag Jnk1a1(apf)

    Plasmid

    #13846

    Depositor

    Roger Davis

    Article

    Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

    Insert

    Jnk1a1(apf) (MAPK8 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced dual phosporylation motif Thr(183)-Pro-T…

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFUW-TetO-Junb

    Plasmid

    #178441

    Purpose

    doxycycline-inducible expression of Mouse Junb in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Junb (Junb Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FKBP-epsin(144-575)-GFP

    Plasmid

    #100733

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    epn1 (Epn1 Mouse)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    clathrin binding middle part aa 144-575

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CD8-mCherry-FRB

    Plasmid

    #100738

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD8A (CD8A Human)
    

    Use

    Tags

    FRB and mCherry

    Expression

    Mammalian

    Mutation

    Truncated

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX Zap70(NC)-SH2

    Plasmid

    #46535

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Zap70(NC) (ZAP70 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-268

    Promoter

    Tac

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCVpuro-Flag-cMyc T58A

    Plasmid

    #20076

    Depositor

    Juan Belmonte

    Article

    Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

    Insert

    cMyc (MYC Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pY002 (pFnCpf1_min)

    Plasmid

    #69975

    Purpose

    Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus without Cas genes nd artificial promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHS0810 pcDNA3.1(+) CMV-SV40 NLS-OgeuIscB-nucleoplasmin NLS-3xHA

    Plasmid

    #176540

    Purpose

    Mammalian expression of nuclear localized OgeuIscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    OgeuIscB
    

    Use

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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