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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,962 results for: pcDNA3.1+

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Showing: 4561 - 4580 of 6962 results

  1. pVH607-Tier1-PCREm4-Nluc

    Plasmid
    #169594
    Purpose
    Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cAMP response element-controlled NanoLuc Luciferase expression cassette
    Expression
    Mammalian
    Promoter
    PCREm4
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169594-plasmid-map-sequence-id-364567

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  4. pcDNA TfR1-miniR

    Plasmid
    #181986
    Purpose
    MiniR genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniE labeled with a reporter
    Depositor
    Kimberly Beatty
    Publication
    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TfR1-MiniR
    Expression
    Mammalian
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    181986-plasmid-map-sequence-id-357263

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  6. FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

    Plasmid
    #137827
    Purpose
    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor
    Depositor
    Joshua Leonard
    Publication
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
    Tags
    3x FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137827-plasmid-map-sequence-id-311923

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  7. pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc

    Plasmid
    #161584
    Purpose
    expression of Myc-tagged kinase-dead mouse Lrrk2 containing D1994S mutation
    Depositor
    Maik Hintze
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lrrk2 (Lrrk2 Mouse)
    Tags
    Myc tag and StrepII
    Expression
    Mammalian
    Mutation
    D1994S
    Promoter
    CMV
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    161584-plasmid-map-sequence-id-318588

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  8. pcDNA3-RsSHC014-RBD-8h

    Plasmid
    #161826
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014
    Depositor
    Erik Procko
    Publication
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T321-D519)
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    161826-plasmid-map-sequence-id-316777

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  9. pcDNA3-Rs7327-RBD-8h

    Plasmid
    #161823
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327
    Depositor
    Erik Procko
    Publication
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T321-D519)
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    161823-plasmid-map-sequence-id-316772

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  12. pcDNA3-sACE2v2(732)-IgG1

    Plasmid
    #154105
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    154105-plasmid-map-sequence-id-304581

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  16. CaMKIV-DCTK75E

    Plasmid
    #126423
    Purpose
    A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)
    Depositor
    Douglas Black
    Publication
    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed lysine 75 to glutamic acid
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126423-plasmid-map-sequence-id-249663

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  19. Opto-M1R

    Plasmid
    #106069
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M1R; Opto-M1R; F11; 722)
    Depositor
    Harald Janovjak
    Publication
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Opto-M1R (CHRM1 Human, Bovine)
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106069-plasmid-map-sequence-id-209711

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  20. hHSF1 K80Q

    Plasmid
    #118351
    Purpose
    Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.
    Depositor
    Lea Sistonen
    Publication
    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hHSF1 (HSF1 Human)
    Tags
    myc and 6xHIS
    Expression
    Mammalian
    Mutation
    K80Q
    Available since
    Nov. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    118351-plasmid-map-sequence-id-227532

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Showing: 4561 - 4580 of 6962 results