We narrowed to 2,342 results for: puc19

1. puC19 preACT1(del6) A259C

   Plasmid

   #111329

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   branch site A259C

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. puC19 preACT1(del6) C256A

   Plasmid

   #111331

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   branch site C256A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19/PLIN2 GFP-P2A-BFP

   Plasmid

   #202195

   Purpose

   knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP-P2A-BFP knock-in

   Expression

   Mutation

   Promoter

   lac

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

   Plasmid

   #223137

   Purpose

   pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-eGFP-P2A-dTAG-Ing3

   Plasmid

   #170754

   Purpose

   Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

   Depositor

   Kristian Helin

   Article

   Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

   Insert

   eGFP-P2A-2xHA-dTAG
   

   Use

   Mouse Targeting

   Tags

   eGFP-P2A-2xHA-dTAG

   Expression

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   none

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-dual-scafffold-2.0 (pBP49)

   Plasmid

   #218931

   Purpose

   The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Insert

   dual scaffolds for CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-747pUC19-tNGFR-P2A-CD7

   Plasmid

   #186086

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CD7 (CD7 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-TgKI-MCS-tdTomato-polyA

   Plasmid

   #174022

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   tdTomato
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKB33 (pUC19-KANMX-tetO7-CYCpr)

   Plasmid

   #210472

   Purpose

   Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Insert

   KanMX-TetO7-CYCpr cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

    Plasmid

    #188634

    Purpose

    HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Human, Synthetic)
    

    Use

    CRISPR

    Tags

    CD3ε-mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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