We narrowed to 9,605 results for: AAT

1. pSMPUW-miR-124-GFP-Puro

   Plasmid

   #117321

   Purpose

   Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   hsa-miR-124-1 (MIR124-1 Human)
   

   Use

   Lentiviral

   Tags

   GFP-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB09-TRE-beta-globin-miR-124-EF1a-GFP

   Plasmid

   #117318

   Purpose

   Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   hsa-miR-124-1 (MIR124-1 Human)
   

   Use

   Transposon-mediated integration

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-inducible promoter

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLCHKOv3-mCherry-intergenic-As

   Plasmid

   #209037

   Purpose

   Lentiviral vector expressing mCherry along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCHKOv3-mClover-intergenic-As

   Plasmid

   #209036

   Purpose

   Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pU6-pegRNA-LRRK2-G2019S-3a

   Plasmid

   #180432

   Purpose

   Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   LRRK2-G2019S

   Promoter

   U6

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pU6-pegRNA-LRRK2-G2019S-3b

   Plasmid

   #180433

   Purpose

   Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   LRRK2-G2019S

   Promoter

   U6

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-pegRNA-LRRK2-G2019S-3c

   Plasmid

   #180434

   Purpose

   Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   LRRK2-G2019S

   Promoter

   U6

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pU6-pegRNA-LRRK2-G2019S-3d

   Plasmid

   #180435

   Purpose

   Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   LRRK2-G2019S

   Promoter

   U6

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6-pegRNA-LRRK2-G2019S-3a

   Plasmid

   #180432

   Purpose

   Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   LRRK2-G2019S

   Promoter

   U6

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-pegRNA-LRRK2-G2019S-3b

    Plasmid

    #180433

    Purpose

    Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    ncRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    LRRK2-G2019S

    Promoter

    U6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-pegRNA-LRRK2-G2019S-3c

    Plasmid

    #180434

    Purpose

    Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    ncRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    LRRK2-G2019S

    Promoter

    U6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-pegRNA-LRRK2-G2019S-3d

    Plasmid

    #180435

    Purpose

    Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    ncRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    LRRK2-G2019S

    Promoter

    U6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLY59

    Plasmid

    #130927

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    sfgfp
    pspFΔHTH::MCP
    

    Use

    Synthetic Biology

    Tags

    ASV tag and MCP (MS2 coat protein)

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-2G6, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. N379K EPHA3 pcDNA3.1

    Plasmid

    #102745

    Purpose

    Expression of EPHA3 in mammalian cells

    Depositor

    Jin Chen

    Article

    Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Tags

    myc-6xhis

    Expression

    Mammalian

    Mutation

    N379K (AAT > AAG)

    Promoter

    CMV

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. T166N EPHA3 pcDNA3.1

    Plasmid

    #102742

    Purpose

    Expression of EPHA3 in mammalian cells

    Depositor

    Jin Chen

    Article

    Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Tags

    myc-6xhis

    Expression

    Mammalian

    Mutation

    T166N (ACT > AAT)

    Promoter

    CMV

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. D806N EPHA3 pcDNA3.1

    Plasmid

    #102754

    Purpose

    Expression of EPHA3 in mammalian cells

    Depositor

    Jin Chen

    Article

    Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Tags

    myc-6xhis

    Expression

    Mammalian

    Mutation

    D806N (GAT > aAT)

    Promoter

    CMV

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. K761N EPHA3 pcDNA3.1

    Plasmid

    #102752

    Purpose

    Expression of EPHA3 in mammalian cells

    Depositor

    Jin Chen

    Article

    Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Tags

    myc-6xhis

    Expression

    Mammalian

    Mutation

    K761N (AAG > AAt)

    Promoter

    CMV

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLY59

    Plasmid

    #130927

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    sfgfp
    pspFΔHTH::MCP
    

    Use

    Synthetic Biology

    Tags

    ASV tag and MCP (MS2 coat protein)

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-2G6, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. N379K EPHA3 pcDNA3.1

    Plasmid

    #102745

    Purpose

    Expression of EPHA3 in mammalian cells

    Depositor

    Jin Chen

    Article

    Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Tags

    myc-6xhis

    Expression

    Mammalian

    Mutation

    N379K (AAT > AAG)

    Promoter

    CMV

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. T166N EPHA3 pcDNA3.1

    Plasmid

    #102742

    Purpose

    Expression of EPHA3 in mammalian cells

    Depositor

    Jin Chen

    Article

    Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

    Insert

    EPHA3 (EPHA3 Human)
    

    Use

    Tags

    myc-6xhis

    Expression

    Mammalian

    Mutation

    T166N (ACT > AAT)

    Promoter

    CMV

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge