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We narrowed to 24 results for: AAT

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Showing: 1 - 20 of 24 results

  1. Plasmid Cloning by PCR

    Type
    Blog Post
    ...site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'....final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...

  2. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...biology education kit." Science Advances 4.8 (2018): eaat5105. PubMed PMID: 30083608. PubMed Central PMCID: ...biology education kit." Science Advances 4.8 (2018): eaat5107. PubMed PMID: 30083609. PubMed Central PMCID: ...

  4. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...genetically encoded sensors." Science 360.6396 (2018): eaat4422. PubMed PMID: 29853555. PubMed Central PMCID: ...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ....1 sequencing primer (5’ CAA GGC TGT TAG AGA GAT AAT TGG A 3’). TIP: You may need to adjust the sequencing... Reverse oligo: 5’ AATTCAAAAA AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT 3’ Back ...antisense—TTTTTG 3’ Reverse oligo: 5’ AATTCAAAAA—21bp sense—CTCGAG—21bp antisense 3’ For example...would be: Forward oligo: 5’ CCGG AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT TTTTTG 3’ Reverse...

  7. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...Science, 361(6397). https://doi.org/10.1126/science.aat0839 Yoshimi, K., & Mashimo, T. (2022). Genome editing...

  8. Plasmids 101: Protein tags

    Type
    Blog Post
    ...EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG  ...

  11. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...can try these new primers: EMX1-Forward: CCATCCCCTTCTGTGAATGT EMX1-Reverse: GGAGATTGGAGACACGGAGA Q16:...

  12. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...BioBrick (RFC 10) Prefix: GAATTC GCGGCCGC T TCTAGA G Alternate Prefix: GAATTC GCGGCCGC T TCTAGA TG (contains...Standard (RFC 25) Prefix: GAATTC GCGGCCGC T TCTAGA TG GCCGGC Alternate Prefix: GAATTC GCGGCCGC T TCTAG (for... 10) Back to Top BioBrick BB-2 (RFC 12) Prefix: GAATTC GCGGCCGC T ACTAGT G Prefix Enzymes: EcoR1 , NotI...Top BglBrick – Berkeley Standard (RFC 21) Prefix: GAATTC ATG AGATCT Prefix Enzymes: EcoR1 , BglII Suffix...21) Back to Top Silver Standard (RFC 23) Prefix: GAATTC GCGGCCGC T TCTAGA G Prefix Enzymes: EcoR1 , NotI...Enzymes: EcoR1 , NotI , XbaI, (NgoMIV) Suffix: ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG Suffix Enzymes: Agel , Spel...

  13. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...OPEN OPEN aCGTTCACTCggtaaTGGGCCGACt Atg7 OZ557 and OZ558 OPEN OPEN tTACTTCTGCaatgatGTGGTGGCTc vipr1 OZ559...CODA CODA tGGCAGCAGCTTTTCGAAGAATAGg zgc:65890 OZ599 and OZ600 OPEN CODA aATCTTCCACAGTGATGAGGACGTGc panx2 ...Encephalopsin (Opn3) OZ541 and OZ542 OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt NFATc1 OZ543 and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc... H3 (hrh3) OZ549 and OZ550 OPEN OPEN gCGCCACGGAcaataaaGAGGACTGCa GUSB OZ551 and OZ552 OPEN OPEN gGTCATCTGCcattcGAGGCGGACa...tGTCTTCATCTCCTTCAGATGCTGTCc fam82a2 OZ573 and OZ574 CODA CODA gCACCGCTGCAGTAAGAGCTGAGGAAt morc3b (NP_001003579.1) OZ575 and OZ576...

  15. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...oligo: 5' - AATTC CATATG TTAATTAA GGCGCGCC CAATTG G - 3' Bottom oligo: 3' - G GTATAC AATTAATT CCGCGCGG GTTAAC...oligo: 5' - CATATG TTAATTAA GGCGCGCC CAATTG - 3' = 28 bp Bottom oligo: 3' - GTATAC AATTAATT CCGCGCGG GTTAAC...tandem ( NdeI - CATATG , PacI - TTAATTAA , AscI - GGCGCGCC , MfeI - CAATTG ). The bottom oligo will be the...' - AATTCCATATGTTAATTAAGGCGCGCCCAATTGG - 3' Bottom oligo: 5' - TCGACCAATTGGGCGCGCCTTAATTAACATATGG - 3'...and SalI (see diagram ). To do this, we add 5' - AATTC and G - 3' to the top oligo and 3' - G and CAGCT...

  16. Immunology Research Plasmids and Resources

    Type
    Collection
    ...TGFBR1 transforming growth factor, beta receptor 1 AAT5, ACVRLK4, ALK-5, ALK5, LDS1A, LDS2A, SKR4, TGFR-...transforming growth factor, beta receptor II (70/80kDa) AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TGFR-2, ...

  17. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...primers: FW 5'–GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication...

  18. AAV Molecular Tools

    Type
    Collection
    ...System Activity Serotype PI 58376 pAAV/D377Y-mPCSK9 hAAT-driven, constitutive Expression of mutant (D377Y...

  19. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'....final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...

  20. Lentivirus ddPCR Titration

    Type
    Protocol
    ...primer: tgtgccttggaatgctagt probe (FAM): tttggaatcacacgacct reverse primer: aatttctctgtcccactccatc PrimePCR...
Showing: 1 - 20 of 24 results