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  1. Plasmid Cloning by PCR

    Type
    Blog Post
    ...site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'....final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...

  2. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...biology education kit." Science Advances 4.8 (2018): eaat5105. PubMed PMID: 30083608. PubMed Central PMCID: ...biology education kit." Science Advances 4.8 (2018): eaat5107. PubMed PMID: 30083609. PubMed Central PMCID: ...

  4. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...genetically encoded sensors." Science 360.6396 (2018): eaat4422. PubMed PMID: 29853555. PubMed Central PMCID: ...

  6. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...Science, 361(6397). https://doi.org/10.1126/science.aat0839 Yoshimi, K., & Mashimo, T. (2022). Genome editing...

  7. Plasmids 101: Protein tags

    Type
    Blog Post
    ...EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG  ...

  8. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ....1 sequencing primer (5’ CAA GGC TGT TAG AGA GAT AAT TGG A 3’). TIP: You may need to adjust the sequencing... Reverse oligo: 5’ AATTCAAAAA AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT 3’ Back ...antisense—TTTTTG 3’ Reverse oligo: 5’ AATTCAAAAA—21bp sense—CTCGAG—21bp antisense 3’ For example...would be: Forward oligo: 5’ CCGG AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT TTTTTG 3’ Reverse...

  9. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGATCTGGATCCTAACAAggatgacttgcccctgtGTGCGAACACCAACCATA arf6 TAL3212 & TAL3213 TGGGGAAGATGCTTTCCAagatcttcggcaacaaGGAGATGAGAATATTGA arnt1 TAL3214 & TAL3215 TCCCGTCTCTGGCCATGAcctcctctaatggatcTCATTCCAACGGTGTGCA... TCCCGTCTCTGGCCATGAcctcctctaatggatcTCATTCCAACGGTGTGCA apoa4 TAL3022 & TAL3025 TGGCAGGACCAACCAATGcccagcatggacctggTGAAAAATGCTTTCTGGA...arap3a TAL3026 & TAL3027 TGGCAACAATCGGTCCGTgctcagatgcagaggaCCTGTTGGCTTCCATCCA ARSA TAL 3002 & TAL 3003... atg12 TAL3216 & TAL3217 TTAATCAGTCGTTTGCGCcatcaccggaccaagaAGTTGGTGTGCTTTTTGA avpl TAL3220 & TAL3221 TGCTGTGTGTGCTCGCGCtctccacgctctcgtcTGCCTGCTACATCCAGAA...casp8ap2 TAL3038 & TAL3039 TATAACAAATCCCACCACattgcccgatatcttgAATACTTCCAAAACAAAA Ccl2 TAL3222 & TAL3223 TTTTCGTTTTCCACTATAaagatcccagttaagcAGGTTCAGAGTTATCATA...cebpd TAL3042 & TAL3043 TGACTTCTGCTGCGTCCAtgtctgacatgtacaatCTGGACTCGCAGTGCGTA CHCHD4 TAL3408 & TAL3409...TAL3409 TCTACCTAATGGCGACATaaactggaactgcccgtGTTTGGGCGGCATGGCCA cish.b-(socs8) TAL3044 & TAL3045 TTCTCAAAATGAGAGGGGtgaatctgaagttcaaCAAACCCAGCAGCACCAA...

  10. Validated gRNA Sequences

    Type
    Collection
    ...sapiens GACTGTAAGTGAATTACTT 69539 cut S. pyogenes 26130722 Yan HPRT1 H. sapiens CTGATAAAATCTACAGTCAT 58778...sapiens CGATGTCACCTCCAATGACT cut S. pyogenes 26789497 Corn CCR5 H. sapiens TGACATCAATTATTATACAT cut S. ...pyogenes 26472758 Sabatini ANT1 S. lycopersicum TGTCGTTTATAATTTGTAGA 70019 cut S. pyogenes 26541286 Voytas APC...pyogenes 24967838 Mashimo ATF1 H. sapiens TAGGAATCAAACACTTTTATTGG 64690 tag S. pyogenes 26355004 Mendenhall...Mendenhall ATM H. sapiens TGAATTGGGATGCTGTTTTT 58779 cut S. pyogenes 24954249 Yamamoto avr-14 C. elegans GATTGGAGAGTTAGACCACG...pyogenes 24879462 Mello avr-15 C. elegans GTTTGCAATATAAGTCACCC 58982 cut S. pyogenes 24879462 Mello AXIN2...25543152 Hanna BT1754 B. thetaiotaomicron GAAAATGGGGTGTATCCTGC 68892 interfere S. pyogenes 26918244 Lu ...

  11. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...BioBrick (RFC 10) Prefix: GAATTC GCGGCCGC T TCTAGA G Alternate Prefix: GAATTC GCGGCCGC T TCTAGA TG (contains...Standard (RFC 25) Prefix: GAATTC GCGGCCGC T TCTAGA TG GCCGGC Alternate Prefix: GAATTC GCGGCCGC T TCTAG (for... 10) Back to Top BioBrick BB-2 (RFC 12) Prefix: GAATTC GCGGCCGC T ACTAGT G Prefix Enzymes: EcoR1 , NotI...Top BglBrick – Berkeley Standard (RFC 21) Prefix: GAATTC ATG AGATCT Prefix Enzymes: EcoR1 , BglII Suffix...21) Back to Top Silver Standard (RFC 23) Prefix: GAATTC GCGGCCGC T TCTAGA G Prefix Enzymes: EcoR1 , NotI...Enzymes: EcoR1 , NotI , XbaI, (NgoMIV) Suffix: ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG Suffix Enzymes: Agel , Spel...

  12. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...OPEN OPEN aCGTTCACTCggtaaTGGGCCGACt Atg7 OZ557 and OZ558 OPEN OPEN tTACTTCTGCaatgatGTGGTGGCTc vipr1 OZ559...CODA CODA tGGCAGCAGCTTTTCGAAGAATAGg zgc:65890 OZ599 and OZ600 OPEN CODA aATCTTCCACAGTGATGAGGACGTGc panx2 ...Encephalopsin (Opn3) OZ541 and OZ542 OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt NFATc1 OZ543 and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc... H3 (hrh3) OZ549 and OZ550 OPEN OPEN gCGCCACGGAcaataaaGAGGACTGCa GUSB OZ551 and OZ552 OPEN OPEN gGTCATCTGCcattcGAGGCGGACa...tGTCTTCATCTCCTTCAGATGCTGTCc fam82a2 OZ573 and OZ574 CODA CODA gCACCGCTGCAGTAAGAGCTGAGGAAt morc3b (NP_001003579.1) OZ575 and OZ576...

  13. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...oligo: 5' - AATTC CATATG TTAATTAA GGCGCGCC CAATTG G - 3' Bottom oligo: 3' - G GTATAC AATTAATT CCGCGCGG GTTAAC...oligo: 5' - CATATG TTAATTAA GGCGCGCC CAATTG - 3' = 28 bp Bottom oligo: 3' - GTATAC AATTAATT CCGCGCGG GTTAAC...tandem ( NdeI - CATATG , PacI - TTAATTAA , AscI - GGCGCGCC , MfeI - CAATTG ). The bottom oligo will be the...' - AATTCCATATGTTAATTAAGGCGCGCCCAATTGG - 3' Bottom oligo: 5' - TCGACCAATTGGGCGCGCCTTAATTAACATATGG - 3'...and SalI (see diagram ). To do this, we add 5' - AATTC and G - 3' to the top oligo and 3' - G and CAGCT...

  14. Cre-lox system

    Type
    Collection
    ...tetracyclin-dependent transgene/shRNA expression ApoE.HCR.hAAT Mammalian Ehmer 85578 pTC-ApoE-Tet CreER expression...tetracyclin-dependent transgene/shRNA expression ApoE.HCR.hAAT Mammalian Ehmer 85797 pSkipFlox split Cre Pfcrt...

  15. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'....final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...

  16. Immunology Research Plasmids and Resources

    Type
    Collection
    ...TGFBR1 transforming growth factor, beta receptor 1 AAT5, ACVRLK4, ALK-5, ALK5, LDS1A, LDS2A, SKR4, TGFR-...transforming growth factor, beta receptor II (70/80kDa) AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TGFR-2, ...

  17. Lentivirus ddPCR Titration

    Type
    Protocol
    ...primer: tgtgccttggaatgctagt probe (FAM): tttggaatcacacgacct reverse primer: aatttctctgtcccactccatc PrimePCR...

  18. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...primers: FW 5'–GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication...

  20. Sequencing Primers

    Type
    Guide
    ...forward primer T3 GCAATTAACCCTCACTAAAGG T3 promoter, forward primer T7 TAATACGACTCACTATAGGG T7 promoter, forward...Pry1 CTTAGCATGTCCGTGGGGTTTGAAT PZ P-element, reverse primer pTrcHis Forward GAGGTATATATTAATGTATCG (Invitrogen...reverse primer T3 GCAATTAACCCTCACTAAAGG T3 promoter, forward primer T7 TAATACGACTCACTATAGGG T7 promoter, forward...primer Tn7-end GGGGTGGAAATGGAGTTTTT Bacterial transposon Tn7 TRC-F CAAGGCTGTTAGAGAGATAATTGGA (Root lab) Human...to 3′. Commonly Used Primers CMV Forward CGCAAATGGGCGGTAGGCGTG (Invitrogen) Human CMV immediate early promoter... forward primer Full Primer List 3'AOX1 GCAAATGGCATTCTGACATCC (Invitrogen) For Pichia vectors with AOX1...AOX1 terminator, reverse primer 5'AOX1 GACTGGTTCCAATTGACAAGC (Invitrogen) For Pichia vectors with AOX1 ...
Showing: 1 - 20 of 23 results