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pAB042b

Plasmid

#79193

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-MBPoff7

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAB042d

Plasmid

#79194

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-p38_2_3

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAB043a

Plasmid

#79222

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-MBP; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

April 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAB082f

Plasmid

#79238

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d23A

Expression

Bacterial

Promoter

Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-H2B-eGFP-SV40-Hygro

Plasmid

#240801

Purpose

Constitutive expression of H2B-eGFP

Depositor

Hans Clevers

Article

Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

Insert

H2B-eGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-OPTN

Plasmid

#208866

Purpose

Stably express GFP-tagged OPTN for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

OPTN (OPTN Human)

Tags

FKBP-GFP

Expression

Mammalian

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Duet_OPTN-thrombin-GST

Plasmid

#216843

Purpose

For expression in E. coli of OPTN (Internal ref. SMC1789)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

Optineurin (OPTN Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-KRAB-MCS_N-term

Plasmid

#136888

Purpose

Doxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

KRAB repressor domain

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-MCS-KRAB_C-term

Plasmid

#136889

Purpose

Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the KRAB repressor domain and a STOP codon

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

KRAB repressor domain

Use

Lentiviral

Tags

None

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG

Plasmid

#139963

Purpose

Over-expression of native 3-hydroxy-3-methyl glutaryl CoA reductase

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

ConjEpi PtEF2_bsd_PttEF2 Pt49202_HMGRVenusThrFLAG_Ptt49202

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMP89b CvTA V124N

Plasmid

#141207

Purpose

Chromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.

Depositor

Francesca Paradisi

Article

Roura Padrosa et al Front Bioeng Biotechnol. 2019 Oct 18;7:2

Insert

CvTA

Expression

Bacterial

Mutation

V124N

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAB049b

Plasmid

#79225

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ HA4-AsiA; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Availability

Academic Institutions and Nonprofits only

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pAB061i10

Plasmid

#79227

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87R); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Availability

Academic Institutions and Nonprofits only

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pAB061i11

Plasmid

#79228

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87K); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Availability

Academic Institutions and Nonprofits only

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pAB061i8

Plasmid

#79231

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87W); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Availability

Academic Institutions and Nonprofits only

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pAB061i9

Plasmid

#79232

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87F); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Availability

Academic Institutions and Nonprofits only

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pAB082c

Plasmid

#79235

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d2A

Expression

Bacterial

Promoter

Ppro1

Availability

Academic Institutions and Nonprofits only

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pAB082d

Plasmid

#79236

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d2B

Expression

Bacterial

Promoter

Ppro1

Availability

Academic Institutions and Nonprofits only

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pAB082e

Plasmid

#79237

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d2C

Expression

Bacterial

Promoter

Ppro1

Availability

Academic Institutions and Nonprofits only

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pAB082g

Plasmid

#79239

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d23B

Expression

Bacterial

Promoter

Ppro1

Availability

Academic Institutions and Nonprofits only

Enlarge