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Showing: 441 - 460 of 2116 results

  1. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

    Plasmid
    #185918
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185918-plasmid-map-sequence-id-367359

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  9. pEh_gRNA1

    Plasmid
    #178758
    Purpose
    Expression of a gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system
    Depositor
    Chase Beisel
    Article
    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2
    Insert
    gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178758-plasmid-map-sequence-id-355197

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  10. pMs_gRNA1

    Plasmid
    #178764
    Purpose
    Expression of a gRNA that targets next to PAM library, used for Marinomonas sp. type I-E system
    Depositor
    Chase Beisel
    Article
    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2
    Insert
    gRNA that targets next to PAM library, used for Marinomonas sp. type I-E system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178764-plasmid-map-sequence-id-355195

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  11. pAc3_gRNA1

    Plasmid
    #178746
    Purpose
    Expression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
    Depositor
    Chase Beisel
    Article
    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2
    Insert
    gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178746-plasmid-map-sequence-id-355198

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  12. pLm_gRNA1

    Plasmid
    #178752
    Purpose
    Expression of a gRNA that targets next to PAM library, used for L. mobilis type I-E system
    Depositor
    Chase Beisel
    Article
    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2
    Insert
    a gRNA that targets next to PAM library, used for L. mobilis type I-E system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178752-plasmid-map-sequence-id-355157

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  13. pAc2_gRNA1

    Plasmid
    #178740
    Purpose
    Expression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
    Depositor
    Chase Beisel
    Article
    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2
    Insert
    gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178740-plasmid-map-sequence-id-355194

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  14. pEPOR1CB0027

    Plasmid
    #117558
    Purpose
    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtADH gene gene in A. thaliana with 1 mismatch at PAM
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    Promoter_AtU6-26 + sgRNA_atADH1{At1G77120) _1mismatch in PAM A. thaliana
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU6-26 (pICSL90002)
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    117558-plasmid-map-sequence-id-228157

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  16. pEF-BFP

    Plasmid
    #138272
    Purpose
    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555
    Insert
    BFP H66 PAM
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H66, PAM motif
    Promoter
    Ef1a
    Available since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    138272-plasmid-map-sequence-id-273617

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Showing: 441 - 460 of 2116 results