We narrowed to 22,469 results for: TIM;

1. pTR-339 pUC19-tNGFR-P2A-TIM3 N

   Plasmid

   #186077

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   TIM3 (HAVCR2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZ005 (AacC2c1 human codon optimized protein)

   Plasmid

   #70171

   Purpose

   Expresses AacC2c1 human codon optimized protein

   Depositor

   Feng Zhang

   Article

   Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

   Insert

   AacC2c1 protein
   

   Use

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV hSyn INTRON HaloTag-STIM1 WPRE

   Plasmid

   #236233

   Purpose

   AAV expression of mouse STIM1 internally tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   HaloTag-Stim1 (Stim1 Mouse)
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV hSyn INTRON mScarlet-STIM2 WPRE

   Plasmid

   #236236

   Purpose

   AAV expression of human STIM2 internally tagged with mScarlet-I

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mScarletI-STIM2 (STIM2 Human)
   

   Use

   AAV

   Tags

   mScarletI in position 29

   Expression

   Mutation

   Signal peptide from STIM1 and residues 15-746 of …

   Promoter

   human Synapsin 1

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV hSyn INTRON mEmerald-STIM1 WPRE

   Plasmid

   #236234

   Purpose

   AAV expression of mouse STIM1 internally tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mEmerald-Stim1 (Stim1 Mouse)
   

   Use

   AAV

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV hSyn INTRON HaloTag-STIM2 WPRE

   Plasmid

   #236235

   Purpose

   AAV expression of human STIM2 internally tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   HaloTag-STIM2 (STIM2 Human)
   

   Use

   AAV

   Tags

   HaloTag in position 29

   Expression

   Mutation

   Signal peptide from STIM1 and residues 15-746 of …

   Promoter

   human Synapsin 1

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-SpCas9(RRTIMR)-P2A-EGFP (AHK149)

   Plasmid

   #223071

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with RRTIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(RRTIMR)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(RRTIMR)=D1135R, S1136R, G1218T, E1219I, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3

   Plasmid

   #221112

   Purpose

   FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   MFA-IgKappa-FAPoptim-STE3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   STE3

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3

   Plasmid

   #221114

   Purpose

   FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   MFA-IgKappa-FAPoptim-STE3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD649-HAsp-TIMD4-COMP5AP-AviTag-9xHis

    Plasmid

    #157295

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    TIMD4 (TIMD4 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

    Plasmid

    #184563

    Purpose

    CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

    Depositor

    Kacper Rogala

    Article

    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

    Insert

    C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    All lysines were mutated to arginines. Codon-opti…

    Promoter

    CMV

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. BICstim-Gag-dCAS9-VPR

    Plasmid

    #120922

    Purpose

    encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

    Depositor

    Théophile Ohlmann, Philippe Mangeot, Emiliano Ricci

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Insert

    GAG (FMLV)-dCAS9-VPR
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEX-SP-YFP-STIM1(23-685)

    Plasmid

    #18857

    Depositor

    Tobias Meyer

    Article

    Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    YFP is inserted between residues 1-23 (signal pep…

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p6610 MSCV-IP N-HAonly TIMP1

    Plasmid

    #35009

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    TIMP1 (TIMP1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3.1 (-) + codon optimized Brn3a

    Plasmid

    #86829

    Purpose

    mammalian expression of the transcription factor Brn3a

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    Brn3a (POU4F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-OptoSTIM1(Cry2(D387A))

    Plasmid

    #70160

    Purpose

    Light-insensitive mutant of OptoSTIM1

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1(Cry2(D387A)) (STIM1 Human, Mustard Weed)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEX-SP-YFP-STIM1(D76A)

    Plasmid

    #18859

    Depositor

    Tobias Meyer

    Article

    Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    D76A mutation - changed aspartate 76 to alanine. …

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pEX-CMV-SP-STIM2(15-746)

    Plasmid

    #18868

    Depositor

    Tobias Meyer

    Article

    Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

    Insert

    STIM2 (STIM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Signal peptide(SP) 1-10 from STIM1 and residues 1…

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEX-SP-CFP-STIM1(23-685)

    Plasmid

    #18858

    Depositor

    Tobias Meyer

    Article

    Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP inserted between residues 1-23 (signal peptid…

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-STIM1-Venus-173-C

    Plasmid

    #87619

    Purpose

    Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    STIM1-Venus173-C (STIM1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge