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Showing: 441 - 460 of 1126 results

  1. dpYPQ230-D156R-CBE1

    Plasmid
    #195367
    Purpose
    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1
    Insert
    hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
    Use
    CRISPR; Gateway compatible ha3a/y130f-xten plus 3…
    Tags
    3xflag
    Expression
    Plant
    Mutation
    Promoter
    ZmUBI1
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    195367-plasmid-map-sequence-id-392890

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  2. pYPQ220-Ultra

    Plasmid
    #176889
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d
    Insert
    AsCas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    176889-plasmid-map-sequence-id-350058

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  3. pRDA_816

    Plasmid
    #201157
    Purpose
    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa
    Depositor
    John Doench
    Article
    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:
    Insert
    5x-ALFA tag-dEnAsCas12a-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D908A
    Promoter
    EFS
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    201157-plasmid-map-sequence-id-396590

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  5. pSLQ10871

    Plasmid
    #183962
    Purpose
    U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    dCas12a(wildtype)
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG and U6
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183962-plasmid-map-sequence-id-363117

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  6. pYPQ230-RV

    Plasmid
    #188544
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RV
    Use
    CRISPR; Gateway compatible lbcas12a-rv entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, R182V
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188544-plasmid-map-sequence-id-369474

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  7. pSLQ10844

    Plasmid
    #183956
    Purpose
    CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    183956-plasmid-map-sequence-id-363081

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  9. pYPQ230-RRV

    Plasmid
    #188543
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRV
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188543-plasmid-map-sequence-id-369473

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  13. pYPQ230-RRVQ

    Plasmid
    #200188
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRVQ
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V, E795Q
    Promoter
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    200188-plasmid-map-sequence-id-396764

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  14. pYPQ230-RVQ

    Plasmid
    #200187
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RVQ
    Use
    CRISPR; Gateway compatible lbcas12a-rv entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, R182V, E795Q
    Promoter
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    200187-plasmid-map-sequence-id-396767

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  15. HEpl017

    Plasmid
    #179992
    Purpose
    pCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh promoter and tagmCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    TadA8e-dSiCas12i(D1049A)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    179992-plasmid-map-sequence-id-358517

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  17. pYPQ230-D156R

    Plasmid
    #188542
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-D156R
    Use
    CRISPR; Gateway compatible lbcas12a-d156r entry c…
    Tags
    NLS
    Expression
    Plant
    Mutation
    D156R
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188542-plasmid-map-sequence-id-369465

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  18. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

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  19. pSLQ10878

    Plasmid
    #183964
    Purpose
    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183964-plasmid-map-sequence-id-363120

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  20. HEpl016

    Plasmid
    #179991
    Purpose
    pCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    SiCas12i
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    179991-plasmid-map-sequence-id-358514

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Showing: 441 - 460 of 1126 results