We narrowed to 901 results for: golden gate assembly
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Plasmid#85984PurposeGolden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoterDepositorInsertNAT
UseGolden gate assemblyPromoterFCPAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Golden Gate TALEN and TAL effector kit 2.0
Plasmid Kit#1000000024PurposeUsed to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.DepositorAvailable SinceJan. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
P18m
Plasmid#120941PurposeMoClo golden gate assembly AB part for T7 promoter (strong, T7 polymerase-dependent constitutive promoter; use with C95m). Please see Supplemental Documents for annotated Genbank file.DepositorInsertT7
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C31m
Plasmid#120966PurposeMoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5. Please see Supplemental Documents for annotated Genbank file.DepositorInsertbxb1 integrase
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P9m
Plasmid#120937PurposeMoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertPr-OR1-OR2
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U9m
Plasmid#121022PurposeMoClo golden gate assembly BC part for BCD22 (very low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD22
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T1m
Plasmid#121028PurposeMoClo golden gate assembly DE part for T500 terminator Engineered terminator derived from bacteriophage 83; see DOI: 10.1126/science.284.5414.611).DepositorInsertT500_noGap, short attachment (T11)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C113m
Plasmid#121012PurposeMoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.DepositorInsertCas9
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U2m
Plasmid#121015PurposeMoClo golden gate assembly BC part for BCD11 (Medium-high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD11
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U3m
Plasmid#121016PurposeMoClo golden gate assembly BC part for BCD13 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD13
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U5m
Plasmid#121018PurposeMoClo golden gate assembly BC part for BCD14 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD14
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6m
Plasmid#121019PurposeMoClo golden gate assembly BC part for BCD20 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD20
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U7m
Plasmid#121020PurposeMoClo golden gate assembly BC part for BCD16 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD16
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U8m
Plasmid#121021PurposeMoClo golden gate assembly BC part for BCD24 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD24
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C84m
Plasmid#120995PurposeMoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.DepositorInsertSigma 28
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C95m
Plasmid#121001PurposeMoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.DepositorInsertT7 RNA polymerase (Andy please check!)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C63m
Plasmid#120979PurposeMoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.DepositorInsertlacI
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C3m
Plasmid#120956PurposeMoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)DepositorInsertsfGFP
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P30m
Plasmid#120942PurposeMoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.DepositorInsertpL-lacO1
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_FLAG3
Plasmid#223896Purpose4G-cloning donor for C-terminal FLAG3 tagDepositorInsertFLAG3
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only