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pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

Plasmid

#182352

Purpose

Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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Tre-Tight ifgMosaic Donor vector (LH121)

Plasmid

#99630

Purpose

To clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaics

Depositor

Rui Benedito

Publication

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox

Mutation

(Please see depositor comments below)

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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YW249_Pzipt17-loxP-H2BGFP-miniMOS

Plasmid

#196063

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

Depositor

Wenxing Yang

Publication

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

H2B::GFP
NeoR
Minimal Mos1
zipt17p::zipt17::zipt17 3UTR

Expression

Worm

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 [282-490]

Plasmid

#186469

Purpose

Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Publication

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [282-490]

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 W201E

Plasmid

#186477

Purpose

Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Publication

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM254 - sgRNA mosTI neoR

Plasmid

#159831

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI neoR

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBACgus_Sumostar/LRRK2 1867-2186_G2019S

Plasmid

#40387

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2186, G2019S

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBACgus_Sumostar/LRRK2 1867-2186_T2031E

Plasmid

#40389

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2186, T2031E

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBACgus_Sumostar/LRRK2 1867-2176

Plasmid

#40392

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2176

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBACgus_Sumostar/LRRK2 1867-2161_T2031E

Plasmid

#40395

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2161, T2031E

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pSEM253 - sgRNA mosTI hygroR

Plasmid

#159827

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI hygroR

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMOS011E: Entry vector for: superecliptic pHluorin (mitochondrial)

Plasmid

#163067

Purpose

Entry vector for: superecliptic pHluorin (mitochondrial)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS017E: Entry vector for: ratiometric pHluorin (cytosolic)

Plasmid

#163069

Purpose

Entry vector for: ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS030: ratiometric pHluorin (endoplasmic reticulum)

Plasmid

#163054

Purpose

ratiometric pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025S

Plasmid

#40394

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2161,C2024S/C2025S

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pTRE3G-marmoset cGASdeltaN-HA

Plasmid

#186863

Purpose

Doxycycline-dependent expression of marmoset cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Marmoset cGAS truncation mutant (160-547 a.a.) with C-terminal HA tag

Use

Retroviral

Tags

HA

Mutation

N-terminal truncation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-marmoset cGAS-HA

Plasmid

#186864

Purpose

Doxycycline-dependent expression of marmoset cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Marmoset cGAS with C-terminal HA tag

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMOSyt1C2AB(80 JuxtaK-421)

Plasmid

#209008

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations

Depositor

Edwin Chapman

Publication

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETSUMOSyt1C2AB(143-421)

Plasmid

#209007

Purpose

Recombinant protein expression of truncated syt1 construct

Depositor

Edwin Chapman

Publication

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 143-421 only

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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MOSMO_pcDNA6.2/EmGFP-Bsd

Plasmid

#176975

Purpose

Mammalian expression vector encoding MOSMO and EmGFP-Bsd

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

MOSMO (C16orf52 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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