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Plasmid#219990PurposeNRAS-5'UTR-NanoLuc mRNA synthesisDepositorInsertNRAS-5'UTR-NanoLuc (NRAS Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-fsr1
Plasmid#179866PurposeEncodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiaeDepositorInsertFusarubin polyketide synthase
UseSynthetic BiologyTagsExpressionMutationCodon optimized for expression in Saccharomyces c…PromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCSh2-mRFP
Plasmid#170982PurposeEncodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCSh2-2300
Plasmid#170984PurposeEncodes sgRNA target 2300 and Kanamycin resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCSh3-2300
Plasmid#170985PurposeEncodes sgRNA target 2300 and Tetracycline resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCSh8-2300
Plasmid#170986PurposeEncodes sgRNA target 2300 and Hygromycin resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC_hU6-shRKIP_BFP
Plasmid#199216PurposeExpression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone.DepositorInsertshBACH1 (BACH1 Synthetic)
UseRNAiTagsExpressionMutationPromoterhuman U6Available sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-Washington
Plasmid#132758PurposeEncodes an iconic representation of a portrait of George WashingtonDepositorInsertWashington1
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-Lincoln
Plasmid#132757PurposeEncodes an iconic representation of a portrait of Abraham LincolnDepositorInsertLincoln1
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-sgNCF1
Plasmid#190193PurposeExpresses a gRNA for base editing the Kozak sequence of the human NCF1 geneDepositorInsertgRNA sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBF120*
Plasmid#162852PurposeExpression of PilB F120 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationF120AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBT125*
Plasmid#162853PurposeExpression of PilB T125 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationT125AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBN189*
Plasmid#162854PurposeExpression of PilB N189 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationN189AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBL193*
Plasmid#162855PurposeExpression of PilB L193 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationL193AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBK197*
Plasmid#162856PurposeExpression of PilB K197 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationK197AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBQ231*
Plasmid#162857PurposeExpression of PilB Q231 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationQ231AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBL240*
Plasmid#162858PurposeExpression of PilB L240 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationL240AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBS249*
Plasmid#162859PurposeExpression of PilB S249 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationS249AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBE309*
Plasmid#162861PurposeExpression of PilB E309 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationE309AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBF486*
Plasmid#162862PurposeExpression of PilB F486 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationF486AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only