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phRL-TKlk-lin-41_LCS-2G

Plasmid

#26544

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

lin-41_LCS-2G

Expression

Mammalian

Mutation

Seed regions of both target sites are mutated.

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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KJBAC1 strain

Bacterial Strain

#13424

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Bacterial Resistance

Tetracycline

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
S4197

Bacterial Strain

#200839

Purpose

wildtype strain MG1655 rph+, ilvG+, ΔlacZ

Depositor

Karin Schnetz

Article

Venkatesh et al J Bacteriol. 2010 Dec;192(24):6456-64. d

Bacterial Resistance

None

Species

Escherichia coli str. k-12 substr. mg1655

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only
rEc2

Bacterial Strain

#51946

Purpose

oriT-kan template

Depositor

Farren Isaacs

Article

Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

Bacterial Resistance

Kanamycin

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only
MCJ.559

Bacterial Strain

#69493

Purpose

Strain with recoded TAG and genomic deletions for improved incorporation

Depositor

Michael Jewett

Article

Hong et al Chembiochem. 2015 Mar 23;16(5):844-53. d

Bacterial Resistance

Ampicillin

Species

Eschericia coli

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
mScarletI3-6DG5-MAPPER

Plasmid

#222421

Purpose

To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

MAPPER

Tags

mScarletI3-6DG5

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6-H2B-mCherry

Plasmid

#89766

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged histone H2B

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

Histone H2B (H2BC11 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRPF215

Plasmid

#106377

Purpose

C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

Depositor

Robert Fagan, Neil Fairweather

Article

Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

Insert

Ptet-himar1-Ter(slpA)-transposon

Use

E. coli - c. difficile shuttle vector

Mutation

Codon optimised for C. difficile

Promoter

Ptet

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.flex.H2B.GFP

Plasmid

#170368

Purpose

Cre-inducible AAV vector expressing nuclear GFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

flex H2B GFP

Use

AAV

Promoter

human Synapsin

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pIRE4-Bpa

Plasmid

#155342

Purpose

Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

Depositor

Irene Coin

Article

Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

Insert

humanized EBpaRS/BstYam

Expression

Mammalian

Promoter

PGK/U6

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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