We narrowed to 7,489 results for: addgene/1000

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pPBneo-CIBN-lin-p53CT(98-393)-R273H-mNeonGreen-NLSx3

Plasmid

#241849

Purpose

Expresses a negative control of the localizer of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

p53 (TP53 Human)

Tags

CIBN and mNeonGreen

Expression

Mammalian

Mutation

p53 C-terminus (97-393 aa) containing R273H mutat…

Promoter

CAGGS promoter

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLOC-D133p53a

Plasmid

#241916

Purpose

Lentiviral vector driving a p53 isoform delta133p53alpha. Stable expression in cultured human cells and possibly cells of other species.

Depositor

Curtis Harris

Article

Ungerleider et al Neuroscience. 2022 Aug 21;498:190-202. d

Insert

Delta133p53alpha (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-N Terminus (pGEX4T1)

Plasmid

#238422

Purpose

Expresses GRP78 N terminus fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-C Terminus (pGEX4T1)

Plasmid

#238423

Purpose

Expresses GRP78 C Terminus fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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STIM1ct-ssrA2-mCerulean

Plasmid

#223690

Purpose

PhoBIT2 component; ssrA2 tag (ssrA mutant A2C) inserted into mCerulean-tagged STIM1 cytoplasmic domain

Depositor

Yubin Zhou

Article

Lee et al Nat Commun. 2025 Jul 28;16(1):6940. doi:

Insert

STIM1(233-685)-ssrA2 (STIM1 Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-T453D

Plasmid

#235209

Purpose

Expresses GRP78 with T453D mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Threonine 453 to Aspartic Acid

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-R197H

Plasmid

#235208

Purpose

Expresses GRP78 with R197H mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Arginine 197 to Histidine

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-G227D

Plasmid

#235207

Purpose

Expresses GRP78 with G227D mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Glycine 227 to Aspartic Acid

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-NLS-Mut

Plasmid

#235206

Purpose

Expresses GRP78 with mutated NLS in mammalian cells

Depositor

Amy Shiu Lee

Article

Liu et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Mutate the NLS sequence

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPGrp

Plasmid

#236002

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to lysosome surface.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Lyso-InPGrp (Cyth3 Rat, Synthetic)

Tags

Cerulean, Full-length LAMP1, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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p70.unipA.NGFR.mCMV.PGK.mBFP2.p27K.WPRE

Plasmid

#228766

Purpose

insert a blue fluorescent protein mBFP2, a cyclin-dependent kinase inhibitor p27K

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Insert

p27 kinase inhibitor (Cdkn1b Mouse)

Tags

mTagBFP2

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pOET3-6xHis-hSMSr

Plasmid

#236224

Purpose

Express N-terminal His-tagged human SMSr (SAMD8) protein in insect cells

Depositor

Chiaki Murakami

Article

Murakami et al J Biol Chem. 2021 Jan-Jun;296:100454. do

Insert

SAMD8 (SAMD8 Human)

Tags

Hexa histidine tag (6xHis-tag) and enterokinase s…

Expression

Insect

Mutation

wild type

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAND1-AM-Tag-PGK-Neo

Plasmid

#223216

Purpose

Targeting plasmid for inserting a C-terminal AM-Tag in the human HAND1 gene

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

HAND1 (HAND1 Human)

Use

Cre/Lox; Human targeting

Tags

AM-Tag

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Nluc-_(PLRSKK)-CA1-19

Plasmid

#232657

Purpose

Nluc-tagged modified CG_ in mammalian cells; lectin-captured luciferase reporter assay

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CGA (CGA Human)

Use

Luciferase

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-EGFR

Plasmid

#222325

Purpose

Synchronize the trafficking of EGFR from the ER.

Depositor

Franck Perez

Article

Scharaw et al J Cell Biol. 2016 Nov 21;215(4):543-558.

Insert

Streptavidin-KDEL and EGFR fused to SBP-EGFP (EGFR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0176 CMVTO-mono-hIL10-R5A11M-mouse_rens-3GS-IL10Ra

Plasmid

#231202

Purpose

CageIL-10-mono (mouse renin cut-site)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

mono-hIL10-R5A11M-mouse_rens-3GS-IL10Ra (IL10 Human)

Expression

Mammalian

Mutation

N36I, N110I, K117N, F129L

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drN-hsAgo2

Plasmid

#221724

Purpose

Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drN-hsAgo2 (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

Swap of N-domain for zebrafish Ago2 N-domain

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsN-drAgo2

Plasmid

#221717

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsN-drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

Swap of N-domain for human Ago2 N-domain

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTag4C_ADRA1A-V2R-NTEV-tcs-GV

Plasmid

#227101

Purpose

ADRA1A receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

ADRA1A-NTEV (ADRA1A Human)

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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p50.mALDH1A1.CD63-GFP.wpre

Plasmid

#226770

Purpose

CD63-GFP in p52 (mAldh1a backbone)

Depositor

Ferdinando Pucci

Article

Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

Insert

CD63 (CD63 )

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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