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Showing: 4601 - 4620 of 11388 results

  1. pcDNA3.3_SARS2_EG.5.1

    Plasmid
    #207641
    Purpose
    pseudovirus plasmid that expresses spike of EG.5.1
    Depositor
    David Nemazee
    Insert
    Spike (EG.5.1) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Q52H, F456L on the basis of XBB.1.5
    Promoter
    CMV
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    207641-plasmid-map-sequence-id-437731

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  2. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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  4. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid
    #195487
    Purpose
    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    C61T, C262V
    Promoter
    T7
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195487-plasmid-map-sequence-id-393190

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  7. pCas9CR5

    Plasmid
    #120227
    Purpose
    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
    Depositor
    Christopher Reisch
    Insert
    SpCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Stop codon inserted before ssrA degradation tag
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120227-plasmid-map-sequence-id-233288

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  8. pJK505

    Plasmid
    #71594
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, additiona…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71594-plasmid-map-sequence-id-131669

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  9. pJK506

    Plasmid
    #71596
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-165 to phenylalanine, addition…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71596-plasmid-map-sequence-id-131670

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  13. pcDNA3.3_SARS2_BA.2.86

    Plasmid
    #208441
    Purpose
    expressing SARS-CoV-2 BA.2.86 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (BA.2.86) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    see GISAID sequence
    Promoter
    CMV
    Available since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    208441-plasmid-map-sequence-id-410934

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  14. pBabe-CD44v6

    Plasmid
    #107970
    Purpose
    expression of CD44 variant
    Depositor
    Georg Weber
    Insert
    mCD44v6 (Cd44 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    splice variant; see Depositor Comments below
    Promoter
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    107970-plasmid-map-sequence-id-218212

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  15. pDON-AI-Npm2(N533)

    Plasmid
    #51475
    Purpose
    Express mouse phosphorylation-mimicking Npm2 (p-Npm, N533)
    Depositor
    Shunsuke Ishii
    Insert
    Nucleoplasmin 2 (Npm2 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Eleven codons (5, 6, 7, 8, 10, 82, 95, 104, 184, …
    Promoter
    Available since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51475-plasmid-map-sequence-id-78547

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  16. pBabe-CD44v4

    Plasmid
    #107969
    Purpose
    expression of CD44 variant
    Depositor
    Georg Weber
    Insert
    mCD44v4 (Cd44 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    splice variant; see Depositor Comments below
    Promoter
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    107969-plasmid-map-sequence-id-222222

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  18. pCas9CR4-VQR

    Plasmid
    #120224
    Purpose
    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015
    Depositor
    Christopher Reisch
    Insert
    SpCas9-VQR
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Mutation
    Mutations D1135V/R1335V/T1337R
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120224-plasmid-map-sequence-id-233296

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  20. pBabe-CD44v7

    Plasmid
    #107971
    Purpose
    expression of CD44 variant
    Depositor
    Georg Weber
    Insert
    mCD44v7 (Cd44 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    splice variant; see Depositor Comments below
    Promoter
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    107971-plasmid-map-sequence-id-212867

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Showing: 4601 - 4620 of 11388 results