We narrowed to 7,027 results for: CAR

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Lenti-N1303K-ngRNA+14_EF1a-puroR

Plasmid

#207359

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Publication

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

N1303K-CFTR +14 ngRNA

Use

Lentiviral

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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GAL4-Creb5 (1- 508)

Plasmid

#195030

Purpose

GAL4-Creb5 fusion construct (full length, 508aa)

Depositor

Andrew Lassar, Cheng-Hai Zhang

Publication

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (full length, 508aa) (CREB5 Bovine)

Expression

Mammalian

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pT2/CMV-eGFP.PB/PTK

Plasmid

#170540

Purpose

Expresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restored

Depositor

Jacob Giehm Mikkelsen

Publication

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

Delta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP

Expression

Mammalian

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Rhodoplanes-bGSDM

Plasmid

#212129

Purpose

For expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.

Depositor

Philip Kranzusch

Publication

Johnson et al Science. 2022 Jan 14;375(6577):221-225.

Insert

Rhodoplanes bGSDM

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Bacteroidetes-bGSDM

Plasmid

#212124

Purpose

For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.

Depositor

Philip Kranzusch

Publication

Johnson et al Science. 2022 Jan 14;375(6577):221-225.

Insert

Bacteroidetes bGSDM

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMO2_Runella-bGSDM

Plasmid

#212123

Purpose

For expression of the bacterial gasdermin (bGSDM) encoded by Runella zeae. Protein is expressed with an N-terminal SUMO2 tag.

Depositor

Philip Kranzusch

Publication

Johnson et al Science. 2022 Jan 14;375(6577):221-225.

Insert

Runella bGSDM

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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tetO-MESP1

Plasmid

#170694

Purpose

doxycycline-inducible overexpression of MESP1

Depositor

Paul Burridge

Publication

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

MESP1 (MESP1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-CES2A-ΔC-S227A

Plasmid

#200556

Purpose

Mammalian expression of secreted mouse CES2A S227A mutant

Depositor

Jonathan Long

Publication

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Tbg-CES2A-ΔC-S227A

Plasmid

#200560

Purpose

Secreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Publication

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)

Use

AAV

Tags

Flag

Promoter

CMV promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-Hook2

Plasmid

#198524

Purpose

Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Publication

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

Hook2 (HOOK2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

His488Gln substitution

Promoter

ADH1

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Pfba1-PYC-Trpl15a

Plasmid

#127738

Purpose

Yeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Synthetic, Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfba1

Available since

Jan. 15, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-CPL2/pET28a

Plasmid

#172095

Purpose

cDNA of CER11/CPL2 cloned into pET28a for protein expression

Depositor

George Haughn

Publication

Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

Insert

CPL2 (CPL2 Mustard Weed)

Tags

6x His

Expression

Bacterial

Promoter

T7

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-FLAG-PGRMC1-1-170

Plasmid

#173467

Purpose

PGRMC1 lacking the extreme C-terminus

Depositor

Bill Tsai

Publication

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deleted for amino acids 171-194

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 P1214R

Plasmid

#166822

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)

Depositor

Daniel Bachovchin

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Mutation

P1214R

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 M77T

Plasmid

#166818

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation M77T (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Mutation

M77T

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 A54T

Plasmid

#166815

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Mutation

A54T

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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ADAP1

Plasmid

#163905

Purpose

Full length mouse ADAP1 for purification from insect cells

Depositor

Martin Loose

Publication

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-FSF1

Plasmid

#110641

Purpose

Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

Depositor

David Sabatini

Publication

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

FSF1 (FSF1 Budding Yeast)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 WWOX WW domain #1

Plasmid

#104235

Purpose

Bacterial expression of WW domain #1 from WWOX

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWOX WW domain #1 (WWOX Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v2 puro

Plasmid

#87793

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Publication

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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