We narrowed to 17,230 results for: emb

1. Opto-ACTU−

   Plasmid

   #198869

   Purpose

   Optogenetic recruitment construct to increase inner membrane surface charge

   Depositor

   Peter Devreotes

   Article

   Banerjee et al Nat Cell Biol. 2022 Oct;24(10):1499-1515

   Insert

   Rad17 polyanionic tail (-14)
   

   Use

   Synthetic Biology

   Tags

   CRY2PHR-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-cMSP1E3D1

   Plasmid

   #199772

   Purpose

   Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

   Insert

   cMSP1E3D1
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Extended cMSP1D1; external His-Tags flanking the …

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNDGG050

   Plasmid

   #231319

   Purpose

   BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG010, pOGG012, and pOGG014, GmR

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEx-deletion-tet

   Plasmid

   #172214

   Purpose

   For allelic exchange in tetracyclin-sensitive Prevotella copri strains

   Depositor

   Till Strowig

   Article

   Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

   Type

   Empty backbone

   Use

   Allelic exchange in prevotella copri

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCherry-mNrp1

   Plasmid

   #21934

   Depositor

   Guido Serini

   Article

   Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

   Insert

   neuropilin 1 (Nrp1 Mouse)
   

   Use

   Tags

   Cherry and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pLV_ADRB2-mTurquoise2-iLID

   Plasmid

   #161002

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   ADRB2-mTurquoise2-iLID
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-MLC2 WT

   Plasmid

   #195235

   Purpose

   Mammalian expression of GFP tagged MLC2. Wild-type

   Depositor

   Keith Mostov, David Bryant

   Article

   Yu et al EMBO Rep. 2008 Sep;9(9):923-9. doi: 10.1

   Insert

   MLC2 (MYL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5HT6-YFP-PIPK

   Plasmid

   #96810

   Purpose

   cilia-membrane-targeted phosphatidylinositol 4-phosphate 5-kinase

   Depositor

   Takanari Inoue

   Article

   Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

   Insert

   5HT6-YFP-PIPK
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C2-GGA2

   Plasmid

   #199172

   Purpose

   Expresses GFP-GGA2 fusion in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   GGA2 (GGA2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Ala 23 Thr substitution; silent substitution in c…

   Promoter

   CMV

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PM-GA

    Plasmid

    #209877

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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